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Evidence for the Role Evolutiona.y Increa.e Yield in Cotton of Tra.sfer Cells in the in Seed a.d Fiber Bioma.s

Molecula. Pla.t 2010年第6期3卷 1075-1086页

作者： deborah a. Pugh Christina.E. Offler Ma.k J. Ta.bot Yong-Ling Rua. School of Environmenta. a.d Life Sciences University of Newcastle Callaghan NSW 2308 Australia a.stra.ia.China.Resea.ch Centre for Crop Improvement University of Newcastle Callaghan NSW 2308 Australia CSIRO Pla.t Industry Canberra ACT 2601 Australia

Tra.sfer cells （TCs） a.e specia.ized cells exhibiting inva.ina.ed wa.l ingrowths （Wls）, thereby a.plifying their pla.ma.membra.e surfa.e a.ea.（PMSa. a.d hence the ca.a.ity to tra.sport nutrients. However, it rema.ns unknown a. to whether TCs pla. a.role in bioma.s yield increa.e during evolution or domestica.ion. Here, we exa.ine this issue from a.compa.a.ive evolutiona.y perspective. The cultiva.ed tetra.loid a. genome species of cotton a.d its a.a.d D genome diploid progenitors displa.ed high, medium, a.d low seed a.d fiber bioma.s yield, respectively. In a.l three species, cells of the innermost la.er of the seed coa. juxta.osed to the filia. tissues tra.s-differentia.ed to a.TC morphology. Electron microscopic a.a.yses revea.ed tha. these TCs a.e cha.a.terized by sequentia. forma.ion of fla.ge a.d reticula.e Wls during the pha.e of ra.id increa.e in seed bioma.s. Significa.tly, TCs from the tetra.loid species developed substa.tia.ly more fla.ge a.d reticula.e Wls a.d exhibited a.higher degree of reticula.e WI forma.ion tha. their progenitors. Consequently, the estima.ed PMSa.of TCs of the tetra.loid species wa. a.out 4 a.d 70 times higher tha. tha. of TCs of the a.a.d D genome progenitors, respectively, which correla.es positively with seed a.d fiber bioma.s yield. Further, TCs with extensive Wls in the tetra.loid species ha. much stronger expression of sucrose syntha.e, a.key enzyme involved in TC Wl forma.ion a.d function, tha. those from the a.a.d D progenitors. The a.a.yses provide a.set of novel evidence tha. the development of TC Wls ma. pla. a. importa.t role in the increa.e of seed a.d fiber bioma.s yield through polyploidiza.ion during evolution.

关键词： Ca.lose cell wa.l cellulose cotton fiber evolution polyploidiza.ion seed development sucrose syntha.e tra.sfer cell.

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Dostoevsky in context

Dostoevsky in context

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作者： (editor.)Ma.tinsen deborah a. (editor.)Ma.虇orova. O. E.

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Novel cla.sifica.ion of ga.tric polyps:The good,the ba. a.d the ugly

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World Journa. of Ga.troenterology 2024年第31期30卷 3640-3653页

作者： deborah Costa.Da.yl Ra.a. a.berto Tringa.i Depa.tment of Digestive Endoscopy a.d Ga.troenterology AULSS2Conegliano HospitalConegliano 31015Italy Depa.tment of Ga.troenterology Division of Hepatology and EndoscopyBrigham and Women's HospitalBostonMA 02115United States

Ga.tric polyps(GPs)a.e increa.ingly *** upper endoscopy,they should be exa.ined with white light a.d occa.iona.ly chromoendoscopy,a.d their morphology cla.sified a.cording to the Pa.is *** GPs ha.e a.typica. endoscopic a.pea.a.ce a.d ca. be a.socia.ed with disea.es like Helicoba.ter pylori *** exa.ina.ion is necessa.y for a. a.cura.e *** most polyps a.e non-neopla.tic a.d do not require trea.ment,some ca.ry a.risk of ma.igna.cy or a.e a.rea.y ***,understa.ding the dia.nosis,cla.sifica.ion,a.d ma.a.ement of GPs is crucia. for pa.ient *** new cla.sifica.ion ca.egorizes GPs into"good","ba.",a.d"ugly"ba.ed on their likelihood of becoming *** a.m to provide descriptions of the endoscopic a.pea.a.ce,pa.hology,trea.ment,a.d follow-up for different GPs,a. well a. clinica. ma.a.ement flowcha.ts.

关键词： Ga.tric polyps Fundic gla.d polyps Hyperpla.tic polyps a.enoma. Neuroendocrine tumors Ea.ly ga.tric ca.cer

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Congenita. Chylothora. Trea.ed with Ora. Sildena.il—Ca.e Report from Nigeria.a.d Review of Litera.ure

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Open Journa. of Pedia.rics 2023年第2期13卷 189-195页

作者： Chinyere C. Uzodimma.Sa.eed B. Sa.ni deborah a. Ma.ise-Wobo Cha.les C. Uzodimma X-Serve Children’s Hospita. Lagos Nigeria Federa. Medica. Centre Abeokuta Nigeria Depa.tment of Pa.dia.rics University of Ottawa Ottawa Canada

Congenita. chylothora., a.condition in which chyle a.cumula.es in the pleura. ca.ity, is extremely ra.e. The reported incidence is 1 in 24,000 births. Medica. trea.ment using octreotide a. well a. chemica. a.d surgica. pleurodesis ha. been reported, but a.so few reports on the successful use of Sildena.il exists. We herein report a.ca.e of congenita. chylothora. referred to our Hospita. a. 7 da.s-old. Sildena.il wa. introduced on da. 6 post intuba.ion. Before the introduction of sildena.il, the pea. tota. da.ly dra.na.e wa. 106 ml/kg/da. (340 mls/da.), a.d a.ter sildena.il wa. introduced, the tota. da.ly dra.na.e dropped progressively to 10 ml/kg/da. by da. 11 post intuba.ion. This ca.e report highlights the successful use of ora. Sildena.il for trea.ment of congenita. chylothora. a.d a.so peculia.ities of ma.a.ement rela.ed to a.resource- constra.ned developing country setting in a.rica.

关键词： Chylothora. Congenita. Sildena.il Children

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a.Simple a.proa.h a.sessing the Vegeta.le Content of a.ia. Ta.eout Mea.s with Nutrition Educa.ion Potentia.

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Food a.d Nutrition Sciences 2012年第3期3卷 296-301页

作者： Christina.M. Polla.d deborah a. Kerr Eliza.eth Edwa.ds Ka.herine a. McNa. Na.a.ie E. Scott a.drea.M. Begley Curtin University Perth Australia.

a.dema.d for convenient ea.ing options ha. led to a. increa.e in foods ea.en a.a. from home, a.d consumers a.e looking for hea.thy options when they ea. out. Increa.ing vegeta.le consumption is a.key public hea.th nutrition prior-ity. a.though a.ia. menu items a.e considered a.hea.thy option, there is little informa.ion a.out their rela.ive hea.th-fulness. This study a.ms to pilot a.simple method for mea.uring the vegeta.le content of popula. a.ia. dishes for use in nutrition educa.ion. Thirty vegeta.le conta.ning ta.e-out dishes from three a.ia. resta.ra.ts (Chinese, Tha. a.d Singa.orea.) in Perth, Western a.stra.ia.were photogra.hed a.d weighted with, a.d without the vegeta.les. Sta.da.d 75 gra. vegeta.le servings per dish a.d the a.era.e vegeta.le content between cuisines were compa.ed. The mea. vegeta.les servings per dish wa. 1.8 (0.25 to 5.5) with no sta.istica. difference between cuisines (p > 0.05). The va.iety a.d a.ount of vegeta.les in individua. dishes va.ied within ea.h cuisine. Vegeta.ia. dishes ha. the highest vegeta.le content a.d noodle a.d rice dishes the lowest (0.23 - 0.75 servings/dish). Digita. ima.ery clea.ly, simply a.d quickly displa.ed the vegeta.le content of a.ia. dishes. The a.ia. ta.e-out dishes were low in vegeta.les. Nutrition educa.ors should encoura.e a.ia. food businesses to increa.e the vegeta.le content of their menus a.d a.vise customers to choose a. lea.t one vegeta.ia. dish.

关键词： Ta.e-a.a. Food Vegeta.les Menu Items

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Rodent ma.a.ement in a.tea.oa.New Zea.a.d: a.proa.hes a.d cha.lenges to la.dsca.e-sca.e control

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Integra.ive Zoology 2024年第1期19卷 8-26页

作者： John G.INNES Gra.t NORBURY a.a.eli Sa.a.IEGO Susa. Wa.KER deborah J.WILSON Ma.a.ki Whenua.La.dca.e Resea.ch HamiltonNew Zealand Ma.a.ki Whenua.La.dca.e Resea.ch AlexandraNew Zealand Ma.a.ki Whenua.La.dca.e Resea.ch AucklandNew Zealand Ma.a.ki Whenua.La.dca.e Resea.ch DunedinNew Zealand

a.tea.oa.New Zea.a.d ha. only four rodent species,a.l *** order of a.riva.,they a.e Pa.ific ra. Ra.tus exula.s,brown ra. ***,house mouse Mus musculus,a.d bla.k ra. *** ma.a.ement in New Zea.a.d a.ms ma.nly to conserve indigenous biodiversity ra.her tha. to protect crops or ma.a.e disea.es,a. is usua. *** describe four ma.or“regimes”a.d one ma.or vision for rodent control in New Zea.a.d to meet ecologica. restora.ion *** cha.lenges for isla.d era.ica.ions a.e for la.ge isla.ds tha. a.e remote or popula.ed by *** 1080 is the only la.ge-sca.e(tens of thousa.ds of hecta.es)option for bla.k ra. control,but its a.plica.ion requires a.justment to counter subsequent ra.id bla.k ra. ***“ecosa.ctua.ies”(mea. 720 ha.use ground-ba.ed tra.s a.d poisons to ta.get ma.nly bla.k ra.s a.d fa.e consta.t *** with ma.ma.-resista.t fences(up to 3500 ha.limit reinva.ion a.d ta.get more pest species a.d ha.e ena.led the return of previously extirpa.ed ta.a.to the ma.n *** Free 2050 a.ms to era.ica.e the ra. species(but not mice)plus some other introduced ma.ma.s from New Zea.a.d by *** vision is not a.ta.na.le with current tools,but resea.ch a.d experimenta. ma.a.ement is exploring techniques a.d *** la.ge sca.e(to 100000 ha.a. which bla.k ra.s a.e now ta.geted for control to extremely low a.unda.ce seems to be unique to New Zea.a.d.

关键词： bla.k ra. brown ra. era.ica.ion house mouse rodent control

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a.systema.ic scoping review of study methodology for ra.domized controlled tria.s investiga.ing probiotics in a.hletic a.d physica.ly a.tive popula.ions

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Journa. of Sport a.d Hea.th Science 2024年第1期13卷 61-71页

作者： a.ex E.Mohr Da.id B.Pyne Geova.a.Silva.Foga.a.Leite deborah a.ins Ja.ie Pugh College of Hea.th Solutions Arizona State UniversityPhoenixAZ 85004USA Resea.ch Institute for Sport a.d Exercise University of CanberraCanberraACT 2617Australia La.ora.ory of Functiona. Fermented Food Faculty of Pharmaceutical SciencesUniversity of São PauloSão Paulo 05508-030Brazil Resea.ch Institute for Sport a.d Exercise Sciences Liverpool John Moores UniversityLiverpool L33AFUK

Ba.kground:The purported ergogenic a.d hea.th effects of probiotics ha.e been a.topic of grea. intrigue a.ong resea.chers,pra.titioners,a.d the la. public *** ha. a.so been a. increa.ed resea.ch focus within the rea.m of sports science a.d exercise medicine on the a.hletic gut ***,compa.ed to other ergogenic a.ds a.d dieta.y supplements,probiotics present unique study *** objectives of this systema.ic scoping review were to identify a.d cha.a.terize study methodologies of ra.domized controlled tria.s investiga.ing supplementa.ion with probiotics in a.hletes a.d physica.ly a.tive ***:Four da.a.a.es(MEDLINE,CINa.L,Cochra.e CENTRa.,a.d Cochra.e Da.a.a.e of Systema.ic Reviews)were sea.ched for ra.domized controlled studies involving hea.thy a.hletes or physica.ly a.tive *** intervention with probiotics a.d inclusion of a.control a.d/or pla.ebo group were *** peer-reviewed a.ticles in English were considered,a.d there were no da.e *** were extra.ted a.d presented in ta.ula. form to deta.l study protocols,cha.a.teristics,a.d *** in ra.domized controlled tria.s wa. determined with the RoB 2.0 ***:a.tota. of 45 studies were included in the review,with 35 using a.pa.a.lel group design a.d 10 using a.cross-over *** ha.f the studies used a.single probiotic a.d the other ha.f a.multi-stra.n *** probiotic dose ra.ged from 2×10^(8)to 1×10^(11)colony forming units da.ly,a.d the length of intervention wa. between 7 a.d 150 *** tha. ha.f the studies directly a.sessed ga.trointestina. symptoms,gut permea.ility,or the gut *** sex ra.io of pa.ticipa.ts wa. hea.ily weighted towa.d ma.es,a.d only 3 studies exclusively investiga.ed ***-level a.verse events were reported in only 2 studies,a.though the methodology of reporting va.ied *** risk of bia. wa. genera.ly low,a.though deta.ls on ra.domiza.ion were la.king in some s

关键词： Exercise Experimenta. methodology Ga.trointestina. symptoms Gut microbiota Probiotic supplementa.ion

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Intera.tions between ma.erna. pa.ity a.d feed a.ditives drive the composition of pig gut microbiomes in the post‑wea.ing period

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Journa. of a.ima. Science a.d Biotechnology 2024年第3期15卷 1241-1257页

作者： Ka.la.La. Edua.do Rosa.Medina.Ga.cia.Cha. Ha.ta. deborah Murra. Pedro E.Urriola.a.dres Gomez Depa.tment of a.ima. Science University of Minnesota1364 Eckles AvenueSaint PaulMN 55108USA New Fa.hion Pork 164 Industrial ParkwayJacksonMN 56143USA

Ba.kground Nursery pigs undergo stressors in the post-wea.ing period tha. result in production a.d welfa.e *** cha.lenges disproportiona.ely impa.t the offspring of primipa.ous sows compa.ed to those of mul-tipa.ous *** is known rega.ding potentia. intera.tions between pa.ity a.d feed a.ditives in the post-wea.ing period a.d their effects on nursery pig ***,the objective of this study wa. to investiga.e the effects of ma.erna. pa.ity on sow a.d offspring microbiomes a.d the influence of sow pa.ity on pig feca. microbi-ome a.d performa.ce in response to a.prebiotic *** wea.ing,piglets were a.lotted into three trea.-ment groups:a.sta.da.d nursery diet including pha.ma.ologica. doses of Zn a.d Cu(Con),a.group fed a.commercia. prebiotic only(Preb)ba.ed on a. a.pergillus oryza. fermenta.ion extra.t,a.d a.group fed the sa.e prebiotic plus Zn a.d Cu(Preb+ZnCu).Results a.though there were no differences in va.ina. microbiome composition between primipa.ous a.d mul-tipa.ous sows,feca. microbiome composition wa. different(R^(2)=0.02,P=0.03).The feca. microbiomes of primipa.ous offspring displa.ed significa.tly higher ba.teria. diversity compa.ed to multipa.ous offspring a. d 0 a.d d 21 post-wea.ing(Pa. d 21(R^(2)=0.03,P=0.04).When a.a.yzing the effects of ma.erna. pa.ity within ea.h trea.ment,only the Preb diet triggered significa.t microbiome distinc-tions between primipa.ous a.d multipa.ous offspring(d 21:R^(2)=0.13,P=0.01;d 42:R^(2)=0.19,P=0.001).Composi-tiona. differences in pig feca. microbiomes between trea.ments were observed only a. d 21(R^(2)=0.12,P=0.001).Pigs in the Con group ga.ned significa.tly more weight throughout the nursery period when compa.ed to those in the Preb+ZnCu *** Nursery pig gut microbiome composition wa. influenced by supplementa.ion with a. a.pergillus oryza. fermenta.ion extra.t,with va.ying effects on performa.ce

关键词： a.pergillus prebiotic Copper Feed a.ditives Ma.erna. Nursery pig microbiome Pa.ity Swine microbiome Tra.e minera.s Zinc

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Binding specificity a.d a.finity a.a.ysis of a. a.ti-protective a.tigen peptide rea.ent using ca.illa.y electrophoresis

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a.va.ces in Bioscience a.d Biotechnology 2014年第1期5卷 40-45页

作者： Joshua.M. Kogot deborah a. Sa.kes Joseph M. Pennington Pa.l M. Pellegrino Dimitra.Stra.is-Cullum Goldbelt Ra.en LLC. Frederick USA US a.my Resea.ch La.ora.ory Adelphi USA

Peptide biosensor rea.ents a.e emerging a. a. a.terna.ive to typica. a.tibody-ba.ed detection methods. Peptides ca. be ra.idly isola.ed using ba.teria. displa. methods for new a.d emerging biothrea.s a.d ca. be chemica.ly synthesized for ra.id, la.ge-sca.e production. With the emergence of peptide biosensor rea.ents, there is a.growing need to develop methods for cha.a.terizing binding intera.tions. Ca.illa.y electrophoresis (CE) is a.free-solution sepa.a.ion method tha. is a.le to determine ta.get a.d a.a.yte binding a.socia.ion (Kb) a.d dissocia.ion consta.ts (Kd). In this study, the Kb, Kd, a.d peptide specificity of a. isola.ed peptide binding rea.ent to protective a.tigen (Pa. of Ba.illus a.thra.is were eva.ua.ed using ca.illa.y electrophoresis a. 10 a.d 20 kV. The rela.ive binding specificity wa. ra.idly a.sessed by mea.uring the peptide rela.ive mobility shift a. 20 kV a. nonequilibrium using bovine serum a.bumin (BSa., horsera.ish peroxida.e (HRP), a.d a. a.ti-Pa.monoclona. a.tibody (ma.). The αPa.peptide wa. shown to be highly specific for Pa. with a.Kd = 177 nM mea.ured a. 20 kV a.d Kd = 312 nM mea.ured a. 10 kV. These results show tha. peptides from ba.teria. displa. libra.ies ca. be ra.idly tested for specificity a.d binding a.finity, in solution, for use a. a.potentia. biosensor rea.ent a.a.nst new a.d emerging biothrea.s.

关键词： a.finity Ca.illa.y Electrophoresis Peptide Biosensor Peptide-Protein Displa. Libra.y

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Objective Phenotyping of Root System a.chitecture Using Ima.e a.gmenta.ion a.d Ma.hine Lea.ning in a.fa.fa.(Medica.o sa.iva.L.)

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Pla.t Phenomics 2022年第1期4卷 342-356页

作者： Zha.you Xu La.ry M.York a.a.d Seethepa.li Bruna.Buccia.elli Ha. Cheng deborah a.Sa.a. USDa.a.S Plant Science Research Unit1991 Upper Buford CircleSt.PaulMN 55108USA Biosciences Division a.d Center for Bioenergy Innova.ion Oak Ridge National LaboratoryOak RidgeTN 37830USA Noble Resea.ch Institute LLCArdmoreOK 73401USA Depa.tment of a.ronomy a.d Pla.t Genetics University of Minnesota1991 Upper Buford CircleSt.PaulMN 55108USA Depa.tment of a.ima. Science University of California2251 Meyer HallOne Shields Ave.DavisCA 95616USA

a.tive breeding progra.s specifica.ly for root system a.chitecture(RSa.phenotypes rema.n ra.e;however,breeding for bra.ch a.d ta.root types in the perennia. crop a.fa.fa.is *** in this a.d other crops for a.tive RSa.breeding ha. mostly used visua. scoring of specific tra.ts or subjective cla.sifica.ion into different root *** ima.e-ba.ed methods ha.e been developed,tra.sla.ion to a.plied breeding is limited.

关键词： breeding Ima.e Root

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