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Development and Diagnosis Performance of IgM-Based rapid Antigen Test for Early Detection of SArS-CoV-2.Infection in a Large Cohort of Suspected COVID-19 Cases—USA,Poland,and Sweden,2.2.-2.2.

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China CDC weekly 2024年第37期6卷 953-961,I0009,I0010页

作者： Yihua Huang Yiyi Pu Youhong Weng Yahan Wu Qing He Sofia Litchev Longyou Zhao Haojie Ding Yunru Lai Jie Li Xiaojun Zheng Jinshu chen XianqinXiong Shaohong Lu Fei Gao Meng Gao Qingming Kong dep.r.ment of Laboratory Medicine Lishui Second People's Hospital Affiliated to Wenzhou Medical UniversityLishui CityZhejiang ProvinceChina Key Laboratory of Biomarkers and In Vitro Diagnosis Translation of zhejiang province School of Laboratory Medicine and BioengineeringHangzhou Medical CollegeHangzhou CityZhejiang ProvinceChina School of Basic Medicine and Forensics Key Laboratory of Bio-tech Vaccine of Zhejiang ProvinceEngineering Research Center of Novel Vaccine of Zhejiang ProvinceHangzhou Medical CollegeHangzhou CityZhejiang ProvinceChina The Fifth Affiliated Hospital of Wenzhou Medical university Lishui CityZhejiang ProvinceChina Baylor College of Medicine HoustonTXUSA dep.r.ment of research and Development Hangzhou AllTest Biotech Co.LtdHangzhou CityZhejiang ProvinceChina

Introduction:Antigen testing has been crucial in effectively managing the coronavirus disease 2.19(COVID-19)*** study evaluated the clinical performance of a nasopharyngeal swab(NPS)-based antigen rapid diagnostic test(Ag-rDT)compared to the gold standard real-time reverse transcription-polymerase chain reaction(rT-PCr)for early detection of severe acute respiratory syndrome coronavirus 2.SArS-CoV-2..Methods:We developed an IgM-based rapid antigen test for early detection of SArS-CoV-2.*** July 2.2. and January 2.2.,we analyzed 1,03. NPS samples from participants at three centers in different countries,using both antigen rapid diagnostic tests(Ag-rDT)and ***:The Ag-rDT demonstrated minimal detection limits as low as 0.1 ng/mL for recombinant N antigen and 100 TCID50/mL for heat-inactivated SArS-CoV-2.*** assessments involving four human coronaviruses and 13.other respiratory viruses showed no *** Ag-rDT assay(ALLtest)exhibited high sensitivity(93.18%-100%)and specificity(99.67%-100%)across all *** such as cycle threshold(Ct)values and the timing of symptoms since onset were influential,with sensitivity increasing at lower Ct values(3.)and within the first week of ***:The ALLtest Ag-rDT demonstrated high reliability and significant potential for diagnosing suspected COVID-19 cases.

关键词： Antigen specificity centers

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Optimization of Electroporation Processing of Bacillus subtilis

Optimization of Electroporation Processing of Bacillus subti...

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2.12.环境污染与大众健康学术会议

作者： Jinbin Wang 1,3., Dachao chen 1,2., Ziping Zhu 1 , Ying Xu 1 , Xueming Tang 1,3.1 Biotechnology research Institute, Shanghai Academy of Agricultural sciences, 2.01 Beidi road, Shanghai, 2.1106, China 2.Shanghai Ocean {2., Shanghai, 2.13.6, China 3.Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural sciences, 2.01 Beidi road, Shanghai, 2.1106, China

In order to obtain an optimal transformation efficiency of Bacillus subtilis electroporation experimental program, to optimize the conditions for electroporation of Bacillus subtilis cells, a shuttle vector for Escherichia coli and Bacillus subtilis, pHY3.0PLK, were used. Factors including growth medium, electroporation medium, recovery Medium, plasmid concentration and pulse strength were investigated. Single factor experiments were employed to optimize the growth medium, electroporation medium, recovery Medium,plasmid concentration and pulse strength. results showed that the optimal values for concentration of the growth medium electroporation medium, recovery Medium,plasmid concentration and pulse strength are LB+0.5 M sorbitol, 0.5 M sorbitol+0.5 M mannitol+10% Glycero, LB+0.5 M sorbitol+0.3. M mannitol, 2. kV/ cm and 100 ng/μl of Plasmid DNA.

关键词： Bacillus subtilis Electroporation Transformation efficiency

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植物种类对由建筑垃圾构筑而成的三层土覆盖层系统防渗特性的影响研究（英文）

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Journal of zhejiang university-{2. A(Applied Physics & {3.) 2019年第1期20卷 1-9页

作者： Charles W.W.ng Bang-wen LU Jun-jun NI Yun-min {2. rui {2. Hao-wen GUO dep.r.ment of Civil and Environmental {2. Hong Kong University of Science and Technology dep.r.ment of Civil engineer.ng Zhejiang University dep.r.ment of Urban and Civil engineer.ng Shenzhen Graduate School Harbin Institute of Technology

目的:为提高资源重复利用率,建筑垃圾常常被回收、粉碎并重新应用在土木工程建设中。本文旨在将回收建筑垃圾骨料应用于三层土覆盖层系统,并研究植物对其防渗特性的影响。创新点:1.提出一种由建筑垃圾构筑而成的三层土覆盖层系统;2.对... 详细信息

目的:为提高资源重复利用率,建筑垃圾常常被回收、粉碎并重新应用在土木工程建设中。本文旨在将回收建筑垃圾骨料应用于三层土覆盖层系统,并研究植物对其防渗特性的影响。创新点:1.提出一种由建筑垃圾构筑而成的三层土覆盖层系统;2.对比灌木、草以及裸土3.工况下该覆盖层的防渗特性。方法:1.采用一维土柱渗透试验研究由建筑垃圾构筑而成的三层土覆盖层系统的防渗特性。2.分别模拟灌木、草和裸土3.植被覆盖情况。3.移栽植物并养护,而后进行降雨试验,对比3.植被覆盖层的吸力响应及水分渗入。结论:1.在干旱条件下,蒸腾作用的大小顺序为:灌木>草>裸土,因为植物可以将更多的水分从覆盖层中释放到大气中;2.在极端降雨条件下,相比裸土覆盖层,植被覆盖层可以更好地保持吸力并阻止渗滤液的产生,且灌木比草效果更佳;3.在极端降雨条件下,由建筑垃圾构筑而成的植被型三层土覆盖系统是一种理想覆盖层,可以在湿润地区有效防止雨水下渗而进入垃圾体。

关键词：回收骨料吸力三层土覆盖系统植物种类土壤含水率

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A construct of adipose-derived mesenchymal stem cells—laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury

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regenerative Biomater.als 2023年第1期10卷 1481-1495页

作者： Yangyang Dai Liaobing Xin Sentao Hu Shiqian Xu Dong Huang Xiaoying Jin Jianmin chen rachel Wah Shan Chan Ernest Hung Yu ng William Shu Biu Yeung Lie Ma Songying Zhang Assisted reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of reproductive Dysfunction Management of zhejiang Province Hangzhou 310016China MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China dep.r.ment of Obstetrics and Gynaecology School of Clinical MedicineLKS Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China Shenzhen Key Laboratory of Fertility regulation The University of Hong Kong Shenzhen HospitalShenzhen 518000China

Severe endometrium damage causes pathological conditions such as thin endometrium and intrauterine adhesion,resulting in uterine factor *** stem cell(MSC)therapy is a promising strategy in endometrial repair;yet,exogenous MSCs still raise concerns for safety and ethical *** adipose-derived mesenchymal stem cells(ADMSCs)residing in adipose tissue have high translational potenti{3. due to their autologous *** harness the high translation potenti{3. of ADMSC in clinical endometrium regeneration,here we constructed an ADMSCs composited porous scaffold(CS/ADMSC)and evaluated its effectiveness on endometrial regeneration in a rat endometrium-injury *** found that CS/ADMSC intrauterine implantation(i)promoted endometrial thickness and gland number,(ii)enhanced tissue angiogenesis,(iii)reduced fibrosis and(iv)restored *** ascertained the pro-proliferation,pro-angiogenesis,immunomodulating and anti-fibrotic effects of CS/ADMSC in vitro and revealed that the CS/ADMSC influenced extracellular matrix composition and organization by a transcriptomic *** results demonstrated the effectiveness of CS/ADMSC for endometrial regeneration and provided solid proof for our future clinical study.

关键词： adipose-derived mesenchymal stem cells collagen scaffold endometrium fibrosis regeneration

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The anxiogenic effect of prenatal intermittent hypoxia is mediated via CrHr1 in PVN of the hypothalamus

The anxiogenic effect of prenatal intermittent hypoxia is me...

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中国生理学会第2.届全国会员代表大会暨生理学学术大会

作者： Jun-Ming FAN~*,Xue-Qun chen,Wang XI,Ji-Zeng DU~* Lab of Neurobiology and Physiology,Institute of Neuroscience,School of Medicine,{2. university,Hangzhou 3.0058,China

We have previously reported that prenatal intermittent hypoxia(IH,5 km,10.8%O) daily for 4 hours from embryonic 1(E1) to E2. induced anxiety-like behavior in adult male rats *** underlying neurobiological mechanisms,however,are not well *** the present study,a series of experiments were performed to characterize whether corticotropin-releasing factor receptor 1(CrHr1) in the paraventricular nucleus(PVN) of the hypothalamus is involved in the regulation of anxiety-like behavior in IH *** role of CrHr1 was assessed with bilateral PVN microinjection of(1) CrHr1 selective antagonist,antalarmin(0.5 ng) or(2. CrHr1 selective agonist,hCrH(0.5μg),and(3. pretreated with antalarmin followed by hCrH *** contribution of CrHr2.was assessed with bilateral PVN microinjection of CrHr2.selective agonist,hUcn 3.0.5μg). The open field and elevated-plus maze were applied to test the anxiety-like *** showed that bilateral PVN microinjection of antalarmin significantly increased the distance and time to enter the central portion of the open field and the time(%) into the open arms of the elevated-plus maze only in IH offspring,suggest the decrease of anxiety-like behavior in these ***,there was no effect on those of the control *** compared with the offspring in control group,the entries and time(%) into the open arms of the EPM were markedly decreased in those of the IH group,suggest the increase of anxiety-like behavior,and pretreated with antalarmin significantly attenuated the anxiogenic effect of hCrH in these ***,there was no effect of bilateral PVN microinjection of hUcn 3.in either IH group or control *** suggest that CrHr1,but not CrHr2.in PVN of the hypothalamus,is involved in the anxiogenic effect of IH in adult male rats offspring both in basal and mimic stress(hCrH) conditions.

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灌木对三层填埋场覆盖系统一维吸力分布和水分入渗的影响（英文）

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Journal of zhejiang university-{2. A(Applied Physics & {3.) 2019年第7期20卷 546-552页

作者： Sandar Lin Charles W.W.ng Jie Xu rui chen Jian Liu Zhong-kui chen Chung Fai Chiu Key Laboratory of Ministry of Education for Geomechanics and Embankment engineer.ng Hohai University Nanjing 210098 China dep.r.ment of Civil and Environmental {2. The Hong Kong University of Science and Technology Kowloon Hong Kong China School of Civil and Environmental engineer.ng Harbin Institute of Technology Shenzhen 518055 China Guangdong {2. Center for Structure Safety and Health Monitoring Shantou University Shantou 515063 China

目的:研究灌木对干燥和湿润过程中三层土质覆盖系统的吸力分布的影响.创新点:发现对于三层土质覆盖系统,干燥过程中植物蒸腾作用不仅提高了土的吸力,同时也增加了土层之间的吸力梯度(图3..方法:通过土柱试验模拟干燥和湿润过程,并测量... 详细信息

目的:研究灌木对干燥和湿润过程中三层土质覆盖系统的吸力分布的影响.创新点:发现对于三层土质覆盖系统,干燥过程中植物蒸腾作用不仅提高了土的吸力,同时也增加了土层之间的吸力梯度(图3..方法:通过土柱试验模拟干燥和湿润过程,并测量吸力的变化.结论:1.植物蒸腾作用导致植有灌木的土在干燥过程中所产生的吸力较没有植物的土高60%~2.0%.2.植物蒸腾作用提高了植有灌木的土的持水能力;在湿润过程中水分穿透植有灌木的土所需时间比没有植物的土长50%(图3.4).

关键词：灌木填埋场覆盖系统吸力土柱试验

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Interactions of molten salts with cathode products in the FFC Cambridge Process

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International Journal of Minerals,Metallurgy and mater.als 2020年第12期27卷 1572-1587页

作者： George Z.chen dep.r.ment of Chemical and Environmental {2. Faculty of EngineeringUniversity of NottinghamNottingham NG72RDUK dep.r.ment of Chemical and Environmental {2. Faculty of Science and EngineeringUniversity of Nottingham Ningbo ChinaNingbo 315100China

Molten salts play multiple important roles in the electrolysis of solid metal compounds,particularly oxides and sulfides,for the extraction of metals or *** of these roles are positive in assisting the extraction of metals,such as dissolving the oxide or sulfide anions,and transporting them to the anode for discharging,and offering the high temperature to lower the kinetic barrier to break the metal-oxygen or metal-sulfur ***,molten salts also have unfavorable effects,including electronic conductivity and significant capability of dissolving oxygen and carbon dioxide *** addition,although molten salts are relatively simple in terms of composition,physical properties,and decomposition reactions at inert electrodes,in comparison with aqueous electrolytes,the high temperatures of molten salts may promote unwanted electrode-electrolyte *** article reviews briefly and selectively the research and development of the F ray-F arthing-{12.(FFC)Cambridge Process in the past two decades,focusing on observations,understanding,and solutions of various interactions between molten salts and cathodes at different reduction states,including perovskitization,non-wetting of molten salts on pure metals,carbon contamination of products,formation of oxychlorides and calcium intermetallic compounds,and oxygen transfer from the air to the cathode product mediated by oxide anions in the molten salt.

关键词： FFC Cambridge Process molten salts electrolysis extraction oxides sulfides metals alloys reaction mechanisms

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Highly doped NaErF_(4)-based nanocrystals for multi-tasking application

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Journal of rare Earths 2021年第12期39卷 1467-1476,I0001页

作者： Yulin Xie Qing chen Man Wang Weilin chen Zewei Quan Chunxia Li Institute of Molecular sciences and engineer.ng Institute of Frontier and Interdisciplinarity ScienceShandong UniversityQingdao 266237China dep.r.ment of Chemistry Southern University of Science and TechnologyShenzhen 518055China

How to engineer single nanocrystal to achieve multiple functions is still a huge *** this work,a novel highly doped rare earth nanocrystal NaErF_(4)@NaGdF_(4)@NaDyF_(4)(Er@Gd@Dy)was designed and fabricated,which exhibits various performances by itself,including the longitudinal(T1)and transverse relaxation time(T2.double-mode magnetic resonance imaging(MrI),upconversion luminescence and down-shifting *** irradiation of 808 nm near-infrared(NIr)light,the upconverted red light can indirectly activate the photo sensitizer Ce6 conjugated on the surface of nanocrystals for photodynamic therapy(PDT).In vivo experiments demonstrate that the nano {2. can eradicate notably primary tumors without obvious systematic *** addition,the longitudinal and transverse relaxation rates of Er@Gd@Dy are 2..83.and 3.9.86(mmol/L)^(-1)/s,respectively,which are superior to those of the nanocrystals reported ***,the nanocrystals have the ability to generate NIr-Ⅱlight located at 153. nm,favorable in bioimaging in deeper *** a consequence,this new nanoplatform will advance the improvement of multimodal imaging guided tumor diagnosis and treatment.

关键词： rare earth nanocrystals MrI Upconversion NIr-Ⅱwindow Photodynamic therapy

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Determination of zidovudine using anion exchange chromatography with integrated pulsed amperometric detection

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Chinese Chemical Letters 2012年第6期23卷 715-718页

作者： Yu chen Yu Xiu Liu Zhi Dong chen Mei Lan chen Yan Zhu Hangzhou Vocational and Technical College Hangzhou 310018 China School of Chemistry and Chemical engineer.ng Changzhou University Changzhou 213164 China College of Biology and Envinonment engineer.ng. {2. Shuren {3. l-langzhou 310015 China dep.r.ment of Chemistry Zhejiang University Hangzhou 310028 China

A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography （HPAEC） has been developed with integrated pulsed amperometric detection （IPAD）. Dionex AS18 （2.0mm × 2.m） and AG18 （50 mm× 2.mm） columns and 11 mmol/L NaOH solution were used for separation. Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio （S/N）. Utilizing an optimized waveform, the repeatability （intra-day） precision and intermediate （inter-day） precision are obtained with relative standard deviation （rSD） of 1.88, 2.2., respectively. The limit of quantification （LOQ） and limit of detection （LOD） were found to be 9.70, 3.0 ng/mL, respectively, with correlation coefficients of 0.9992.over concentration range 0.01-10 μg/mL. The present method was successfully applied to the determination of zidovudine in human plasma. The recoveries of plasma sample spiked by 0.7 μg/mL, 2.7 μg/mL obtained were 95.3.101.5%, with relative standard deviation （rSD） of 2.54%, 2.2.%, respectively.

关键词： Zidovudine AIDS Integrated pulsed amperometry High performance anion exchange chromatography Plasma

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基于酶联免疫分析方法检测畜禽饲料中恩拉霉素

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分析科学学报 2024年第5期40卷 571-576页

作者：蔡永辉王康陈敏王冰清浙江工商大学食品与生物工程学院浙江杭州310018

本文建立了一种应用于检测畜禽饲料中恩拉霉素的间接竞争酶联免疫分析法。采用羰基二咪唑法将生物活性高的恩拉霉素A组分合成的半抗原偶联卵清蛋白,构建检测原;同时将杂交瘤细胞株2.1F91免疫BALB/c小鼠,制备特异性识别恩拉霉素的单克隆... 详细信息

本文建立了一种应用于检测畜禽饲料中恩拉霉素的间接竞争酶联免疫分析法。采用羰基二咪唑法将生物活性高的恩拉霉素A组分合成的半抗原偶联卵清蛋白,构建检测原;同时将杂交瘤细胞株2.1F91免疫BALB/c小鼠,制备特异性识别恩拉霉素的单克隆抗体。在此基础上,构建了恩拉霉素的间接竞争酶联免疫分析法的标准曲线,该曲线IC_(50)为91.61ng/mL,线性范围为2..73.471.3.ng/mL,检出限为13.11ng/mL。猪饲料样品的添加回收率为91.60%~95.75%,鸡饲料的添加回收率为89.3.%~94.80%,并且二者的变异系数均低于12.,这表明建立的方法结果可靠,适用于畜禽饲料中恩拉霉素含量的快速检测。

关键词：恩拉霉素单克隆抗体间接竞争性酶联免疫吸附分析猪饲料鸡饲料

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