Determination of zidovudine using anion exchange chromatography with integrated pulsed amperometric detection

作     者：Yu Chen Yu Xiu Liu Zhi Dong Chen Mei Lan Chen Yan Zhu 

作者机构：Hangzhou Vocational and Technical College Hangzhou 310018 China School of Chemistry and Chemical Engineering Changzhou University Changzhou 213164 China College of Biology and Envinonment Engineering. Zhejiang Shuren University l-langzhou 310015 China Department of Chemistry Zhejiang University Hangzhou 310028 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2012年第23卷第6期

页      面：715-718页

核心收录：

学科分类：080904[工学-电磁场与微波技术] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0810[工学-信息与通信工程] 081105[工学-导航、制导与控制] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0703[理学-化学] 081001[工学-通信与信息系统] 081002[工学-信号与信息处理] 0825[工学-航空宇航科学与技术] 0811[工学-控制科学与工程] 081102[工学-检测技术与自动化装置] 

基　　金：supported by Zhejiang Provincial Natural Science Foundation of China(No.Y2110945) Zhejiang Provincial Analysis and Testing Foundation of China(No.2011C37065) 

主　　题：Zidovudine AIDS Integrated pulsed amperometry High performance anion exchange chromatography Plasma 

摘      要：A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography （HPAEC） has been developed with integrated pulsed amperometric detection （IPAD）. Dionex AS18 （250mm × 2mm） and AG18 （50 mm× 2 mm） columns and 11 mmol/L NaOH solution were used for separation. Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio （S/N）. Utilizing an optimized waveform, the repeatability （intra-day） precision and intermediate （inter-day） precision are obtained with relative standard deviation （RSD） of 1.88, 2.27, respectively. The limit of quantification （LOQ） and limit of detection （LOD） were found to be 9.70, 3.0 ng/mL, respectively, with correlation coefficients of 0.9992 over concentration range 0.01-10 μg/mL. The present method was successfully applied to the determination of zidovudine in human plasma. The recoveries of plasma sample spiked by 0.7 μg/mL, 2.7 μg/mL obtained were 95.3-101.5%, with relative standard deviation （RSD） of 2.54%, 2.21%, respectively.