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解毒消癥饮抑制大肠癌细胞EMT及PD-L1表达相关研究

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山西中医 2024年第3期40卷 53-56页

作者：冯海兰陈虹陈旭征魏丽慧方翌张竹青曹治云福建中医药大学福州350122

目的:从上皮间质转化(EMT)及免疫检查点配体-1 (PD-L1)角度探讨解毒消癥饮治疗大肠癌的生物学机制。方法:从TCGA数据库收集267例大肠癌患者相关数据,采用生物信息学分析大肠癌患者肿瘤特异性生存期与EMT和PD-L1的相关性;采用热图分析EM... 详细信息

目的:从上皮间质转化(EMT)及免疫检查点配体-1 (PD-L1)角度探讨解毒消癥饮治疗大肠癌的生物学机制。方法:从TCGA数据库收集267例大肠癌患者相关数据,采用生物信息学分析大肠癌患者肿瘤特异性生存期与EMT和PD-L1的相关性;采用热图分析EMT相关转录因子与PD-L1之间的相关性,划痕实验检测解毒消癥饮对大肠癌细胞HCT-15和HCT-116愈合能力的影响,Western blot实验检测解毒消癥饮对大肠癌细胞HCT-15和HCT-116 EMT相关因子ZEB1、Snail、PD-L1、ZO-1和E-cadherin等蛋白表达的影响。结果:高表达EMT和高表达PD-L1显著降低了CRC患者生存期(P<0.01,P<0.05);PD-L1基因的表达与EMT相关因子基因(TWIST1、TWIST2、ZEB1、ZEB2、Snail1、Snail2、Vimentin等)表达呈正相关;解毒消癥饮干预后的大肠癌细胞愈合能力显著降低(P<0.05);同时,解毒消癥饮抑制肠癌细胞中EMT因子蛋白ZEB1、Snail、PD-L1的表达,上调ZO-1和E-cadherin蛋白的表达。结论:解毒消癥饮抑制肠癌EMT和免疫检查点配体PD-L1表达,对肠癌的抑制作用与抗肿瘤转移和调节免疫微环境相关。

关键词：大肠癌解毒消癥饮 EMT PD-L1 实验研究

Application of Serum Pharmacology in Evaluating the Antitumor Effect of Fuzheng Yiliu Decoction(扶正抑瘤方) from Chinese Medicine

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Chinese Journal of Integrative Medicine 2014年第6期20卷 450-455页

作者：陈旭征曹治云廖联明刘志臻杜建 Academy of Integrative Medicine Fujian University of Traditional Chinese Medicine The Second Affiliated Hospital Fujian University of Traditional Chinese Medicine

Objective： To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine （CM） of Fuzheng Guben （扶正固本, supporting the healthy energy and strengthening the body＇s resistance to pathogens）, the effects of Fuzheng Yiliu Decoction （扶正抑瘤方, FYD）, a typical prescription of Fuzheng Guben, on proliferation and apoptosis of hepatoma cells in vitro were observed by two methods with serum pharmacology and traditional pharmacology, respectively. Methods： HepG2 cells were treated with FYD- containing serum or crude FYD extract in vitro. The proliferation rate was determined by methyl thiazolyl tetrazolium （MTT） assay. Cell cycle and apoptosis rate was performed by flow cytometry. And the levels of interleukin-2 （IL-2） and tumor necrosis factor α （TNF-α） in FYD-containing serum were detected by radioimmunoassay. Results： FYD-containing serum remarkably inhibited proliferation and induced apoptosis of hepatoma cells at least by promoting the production of IL-2 and TNF- α in vivo. On the contrary, crude FYD extract promoted the proliferation and did not induce cell apoptosis. Conclusion： The results by serum pharmacology were accordant with those of our previous animal and clinical trials which indicates that serum pharmacology is a reasonable and feasible method for the evaluation of the antitumor effect of herbs of Fuzheng Guben.

关键词： Fuzheng Guben Chinese medicine serum pharmacology hepatoma

康欣胶囊及其拆方对肾虚血瘀型大鼠神经细胞的保护作用

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中医杂志 2007年第5期48卷 453-456页

作者：蔡晶杜建刘锡棐陈旭征福建中医学院福州市五四路282号350003 南京军区南京总医院神经内科

目的探讨康欣胶囊全方及其补肾、健脾、养血活血3组拆方对肾虚血瘀型血管性痴呆(VD)大鼠神经细胞的影响。方法采用双侧椎动脉永久性封闭和摘除单侧性器官建立肾虚血瘀型VD模型大鼠,分别给予康欣胶囊全方(全方组)、康欣胶囊补肾方(补肾组... 详细信息

目的探讨康欣胶囊全方及其补肾、健脾、养血活血3组拆方对肾虚血瘀型血管性痴呆(VD)大鼠神经细胞的影响。方法采用双侧椎动脉永久性封闭和摘除单侧性器官建立肾虚血瘀型VD模型大鼠,分别给予康欣胶囊全方(全方组)、康欣胶囊补肾方(补肾组)、康欣胶囊健脾方(健脾组)、康欣胶囊养血活血方(养血活血组)、阳性对照药喜得镇(西药组)和蒸馏水(模型组)。结果全方组大鼠空间探索能力显著优于其他组(P<0.01);全方组海马神经细胞器及细胞核固缩情况轻于其他各组;全方组能增加海马乙酰胆碱含量(P<0.01),提高VD大鼠海马酪氨酸激酶A、核转录因子、原癌基因C-fos、神经生长因子受体的表达(P<0.01),降低胶质纤维酸性蛋白的表达(P<0.05)。结论康欣胶囊全方对肾虚血瘀型血管性痴呆大鼠神经细胞具有保护作用。

关键词：康欣胶囊肾虚血瘀血管性痴呆酪氨酸激酶A 核转录因子 C-fos 神经生长因子受体胶质纤维酸性蛋白

Bear Bile Powder(熊胆粉) Induces Apoptosis of Human Hepatocellular Carcinoma Cells via Mitochondrion-Dependent Pathway

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Chinese Journal of Integrative Medicine 2014年第2期20卷 123-129页

作者：赵锦燕陈志鸿林薇钟小勇陈旭征彭军洪振丰 Fujian Academy of Integrative Medicine Fujian Key Laboratory of Integrative Medicine on Geriatrics Fujian University of Traditional Chinese Medicine Fujian Guizhentang Pharmaceutical Co. Ltd.

Objective： To evaluate the effect of Bear Bile Powder （熊胆粉, BBP） on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells, and investigate the possible molecular mechanisms mediating its anti-cancer activity. Methods： HepG2 cells were treated with 0.4-1.0 mg/mL of BBP for 24, 48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5,5＇,6,6＇-tetrachloro- 1 ,1＇,3,3＇-tetraethyl-benzimidazol-carbocyanine iodide （JC-1） staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay. Results： The treatment with 0.4-1 mg/mL of BBP for 24, 48, or 72 h respectively reduced cell viability significantly by 7%-60%, 20%-90% or 25%-98%, compared with the untreated control cells （P〈0.01）. In addition, BBP treatment induced morphological changes in HepG2 cells. Furthermore, after treated with 0, 0.4, 0.6, 0.8 and 1.0 mg/mL of BBP, apoptosis cells （including early and late apoptotic cells） were 18.0% ± 1.3%, 34.9% ± 2.2%, 33.9% ± 2.8%, 37.4% ± 2.8% and 46.0% ± 2.5%, respectively （P〈0.05）; and the percentage of cells with reduced JC-1 red fluorescence were 6.6% ± 0.8%, 8.5% ± 0.8%, 13.5% ± 1.6%, 17.6%± 2.3% and 46.7% ± 3.6%, respectively （P〈0.01）. Finally, BBP treatment significantly and dose-dependently induced activation of both caspase-9 and caspase-3 in HepG2 cells （P〈0.05）. Conclusions： BBP could inhibit the growth of HepG2 hepatocellular cancer cells through mitochondrion-mediated apoptosis, which may, in part, explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.

关键词： Bear Bile Powder hepatocellular carcinoma apoptosis mitochondria

血府逐瘀汤影响小鼠骨髓造血干细胞的实验研究

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中国中西医结合杂志 2007年第6期27卷 527-530页

作者：高冬林久茂郑良朴林薇陈旭征宋军陈可冀福建中医学院中国中医科学院医学实验中心中国中医科学院西苑医院

目的观察血府逐瘀汤对小鼠骨髓造血干细胞的数量、表型、细胞周期和集落形成能力的影响。方法昆明小鼠随机分为对照组及血府逐瘀汤高(13g/kg)、中(6.5g/kg)、低剂量(3.25g/kg)组,灌胃给药7天后,分离骨髓造血干细胞,进行细胞计数,流式细... 详细信息

目的观察血府逐瘀汤对小鼠骨髓造血干细胞的数量、表型、细胞周期和集落形成能力的影响。方法昆明小鼠随机分为对照组及血府逐瘀汤高(13g/kg)、中(6.5g/kg)、低剂量(3.25g/kg)组,灌胃给药7天后,分离骨髓造血干细胞,进行细胞计数,流式细胞仪检测造血干细胞表型Sca和细胞周期,并采用甲基半固体培养基检测集落的形成。结果各组小鼠骨髓单核细胞和悬浮细胞数量以及集落产率差异无显著性(P>0.05),但低剂量组CD34和Sca-1的表达提高(P<0.05),中剂量组只能明显增加Sca-1的表达(P<0.05)。结论血府逐瘀汤通过增加较早期造血干细胞的数量和功能,提高造血能力,从而达到去瘀生新的作用。

关键词：血府逐瘀汤造血干细胞骨髓

Fuzheng Yiliu Granule(扶正抑瘤颗粒)Inhibits the Growth of Hepatocellular Cancer by Regulating Immune Function and Inducing Apoptosis In Vivo and In Vitro

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Chinese Journal of Integrative Medicine 2011年第9期17卷 691-697页

作者：曹治云陈旭征廖联明彭军胡海霞刘志臻杜建 Academy of Integrative Medicine Fujian University of Traditional Chinese Medicine

Objective：To study the inhibitory effect of Fuzheng Yiliu Granule（扶正抑瘤颗粒,FYG） on hepatocellular cancer（HCC） and investigate the mechanism mediating its ***：H22 tumor-bearing ICR mice were treated with FYG[3.6 g/（kg·d）]for 5 *** volume and tumor weight,percentages of CD3~＋,CD4~＋,CD8~＋,and natural killer（NK） cells in peripheral blood,tumor apoptosis and serum levels of interleukin-2（IL-2）,and tumor necrosis factor-α（TNF-α） were ***-containing serum was prepared from SD rats treated for 7 days[high dose 3.6 g/（kg·d）;middle dose 1.8 g/（kg·d）;low dose 0.9 g/（kg·d）].Cell cycle,cell viability,and apoptosis were evaluated after HepG2 cell line was cultured in FYG-containing serum for 48 *** levels of IL-2 and TNF-αin FYG-containing serum were also ***：FYG produced a potent antitumor effect（P0.01） and induced marked apoptosis of the tumor tissue（P0.05）.Mice treated with FYG had higher percentages of CD3~＋ and CD4~＋（P0.05）,and more NK cells（P0.01） in the peripheral blood than those in the animals treated with normal *** receiving FYG had the highest serum levels of IL-2 and TNF-α（P0.01）.High-dose FYG-containing serum significantly decreased HepG2 cell viability,inhibited cell proliferation（P0.05）,and induced apoptosis（P0.01）.In addition,the levels of IL-2 and TNF-αof highdose -containing serum were higher than the blank serum（P0.01）.Conclusion：FYG could inhibit HCC growth by regulating immune function and inducing apoptosis of tumor cells in vivo and in vitro.

关键词： Fuzheng Yiliu Granule hepatocellular cancer immune function apoptosis

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田基黄对人肝癌细胞HepG2增殖的影响

中药药理与临床 2007年第5期23卷 136-137页

作者：林久茂王瑞国陈旭征赵锦燕福建中医学院中西医结合研究院福建中医学院药学系福州350108

目的:探讨田基黄对人肝癌细胞增殖的影响。方法:体外培养人肝癌细胞株HepG2,用MTT法和流式细胞术(FCM)检测田基黄提取物及其含药血清作用于HepG2 24h后的细胞增殖情况。结果:MTT法结果表明,田基黄对HepG2增殖有抑制作用;FCM检测表明,田... 详细信息

目的:探讨田基黄对人肝癌细胞增殖的影响。方法:体外培养人肝癌细胞株HepG2,用MTT法和流式细胞术(FCM)检测田基黄提取物及其含药血清作用于HepG2 24h后的细胞增殖情况。结果:MTT法结果表明,田基黄对HepG2增殖有抑制作用;FCM检测表明,田基黄阻止增殖中的HepG2进入S期。结论:田基黄能有效地抑制HepG2细胞增殖,提示阻滞细胞周期、抑制细胞增殖可能是田基黄治疗肝癌的一个机制。

关键词：田基黄肝癌 HepG2 细胞增殖细胞周期

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衰老对小鼠骨髓造血干细胞影响的实验研究

中国老年学杂志 2009年第8期29卷 946-948页

作者：高冬林薇林久茂郑良朴陈旭征宋军陈可冀福建中医学院中西医结合系福建福州350108 中国中医科学院医学实验中心中国中医科学院西苑医院

目的观察机械刺激下,衰老对小鼠骨髓造血干细胞的数量、表型、细胞周期和集落形成能力的影响。方法分离老年和幼年昆明小鼠的骨髓造血干细胞,进行细胞计数;流式细胞仪检测细胞表型和细胞周期;并采用甲基半固体培养基检测集落的形成。结... 详细信息

目的观察机械刺激下,衰老对小鼠骨髓造血干细胞的数量、表型、细胞周期和集落形成能力的影响。方法分离老年和幼年昆明小鼠的骨髓造血干细胞,进行细胞计数;流式细胞仪检测细胞表型和细胞周期;并采用甲基半固体培养基检测集落的形成。结果两组小鼠骨髓单核细胞和悬浮细胞数量无显著差异,但幼年组细胞Sca-1和CD34的表达较高,体外培养时易形成集落,且CFU-Mix集落产率较老年组有显著差异,说明机械刺激易激活幼年小鼠骨髓中的造血干细胞,使其数量增多,增殖能力增强。结论衰老影响小鼠骨髓中造血干细胞对机械刺激的反应,降低其数量、功能和增殖能力。

关键词：衰老造血干细胞骨髓

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芎芍胶囊对人脐静脉内皮细胞增殖的影响

中药药理与临床 2009年第1期25卷 51-53页

作者：林久茂赵锦燕王瑞国庄群川陈旭征福建中医学院中西医结合研究院福州350108 福建中西医结合研究院福州350108 福建中医学院药学系福州350108

目的:研究芎芍胶囊对人脐静脉内皮细胞(HUVEC)增殖的影响。方法:采用血清药理学和体外培养HUVEC的方法,通过MTT法及流式细胞术(FCM)检测芎芍胶囊含药血清对细胞增殖能力的影响。结果:MTT法检测HUVEC在芎芍胶囊浓度5%时产生最大增殖效应... 详细信息

目的:研究芎芍胶囊对人脐静脉内皮细胞(HUVEC)增殖的影响。方法:采用血清药理学和体外培养HUVEC的方法,通过MTT法及流式细胞术(FCM)检测芎芍胶囊含药血清对细胞增殖能力的影响。结果:MTT法检测HUVEC在芎芍胶囊浓度5%时产生最大增殖效应,增殖率为54.98%;FCM法检测发现芎芍胶囊浓度为5%时S期比例最大,为42.65%。当浓度达10%及以上时,其促HUVEC增殖的作用逐渐减弱。结论:芎芍胶囊能促进HUVEC的增殖,在一定浓度内,其促进细胞增殖的作用与浓度有一定的关系;提示芎芍胶囊的促血管新生的作用可能是通过促进血管内皮细胞的增殖。

关键词：芎芍胶囊人脐静脉内皮细胞细胞增殖