检索结果-南通市图书馆

Inhibition of caspases and intracellular free Ca2+ concentrations are involved in resveratrol protection against apoptosis in rat primary neuron cultures

引用

Acta Pharmacologica Sinica 2007年第11期28卷 1724-1730页

作者： Qi-hai GONG~(2,3,4),Qian WANG~(2,3),Jing-shan SHI~(2,3),Xie-nan HUANG~(2,3),Qiong LIU~2,Hu MA~2~2 Department of Pharmacology,Zunyi Medical College,Zunyi 563000,China ~3 the Key Laboratory of Basic Pharmacology of GuizhouProvince,Zunyi Medical College,Zunyi 563000,China Department of Pharmacology Zunyi Medical College Zunyi China Key Laboratory of Basic Pharmacology of Guizhou Province Zunyi Medical College Zunyi China

Aim:To investigate the influence of resveratrol （Res）,a nutritional antioxidant,onthe inhibition of apoptosis in rat primary neuron ***:The culturedcortical neurons of neonatal Sprague-Dawley rats were pretreated with Res （0.1,1.0,and 10.0 μmol/L） and oxygen-glucose deprivation/reperfusion （OGD/RP） withoxygen and glucose were initiated at d 10 in *** apoptosis wasdetermined by flow cytometry,and morphological changes of neurons wereobserved by an electron *** the mechanism studies,the intracellularfree calcium concentration ([Ca2+]i) and the transcription of caspases-3 and-12 inneurons were detected by Fura 2.aM loading and real-time RT-PCR,***:OGD/RP insult could induce an increase in the apoptotic rate of neurons（from 11.1% to 49.0%）,and elicit an obvious morphological change in neurons;pretreatments with Res （0.1,1.0,and 10.0 μmol/L,respectively） significantlyreduced the elevated rate of apoptosis to 41.7%,40.8%,and 37.4%,respectively,and ameliorated the neuronal morphological ***,the OGD/RP insultobviously elicited the elevated levels of the [Ca2+]iand the expressions of caspases-3and-12 mRNA in *** pretreatments markedly depressed the neuronalabnormal elevation of [Ca2+]iand the overexpression of caspases-3 and-12 mRNAin a concentration-dependent ***:Res can attenuate the ratcortical neuronal apoptosis induced by OGD/*** mechanisms are,at leastpartly,due to the inhibition of the calcium overload and the overexpression ofcaspases-3 and-12 mRNA.

关键词： resveratrol oxygen-glucose deprivation/reperfusion brain ischemia apoptosis neurons

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cDNA Expression Array Analysis of Gene Expression in Human Hepatocarcinoma Hep3B Cells Induced By BNIPL-1

引用

Acta Biochimica et Biophysica Sinica 2005年第9期37卷 618-624页

作者： Li XIE~(1,2) Wen-Xin QIN~(2,3*) Jin-Jun LI~(2,3) Xiang-Huo HE~1 Hui-Qun SHU~2 Gen-Fu YAO~2 Da-Fang WAN~(2,3*) Jian-Ren GU~~(2,3) 1 Shanghai Medical College,Fudan University,Shanghai 200032,China 2 National Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032,China 3 Cancer Institute,Shanghai Jiaotong Universiry,Shanghai 200032,China Shanghai Medical College Fudan UniversityShanghai 200032 China National Laboratory for Oncogenes and Related Genes Shanghai Cancer InstituteShanghai 200032 China Cancer Institute Shanghai Jiaotong UniversityShanghai 200032 China

Bcl-2.adenovirus E1B 19 kDa interacting protein 2 like-1 （BNIPL-1） is a novel human proteinidentified in our laboratory,which can interact with Bcl-2.and Cdc42.aP and induce apoptosis via theBNIP-2.and Cdc42.aP homology （BCH） *** the present study,we established the Hep3B-Tet-onstable cell line in which expression of BNIPL-1 can be induced by *** cell proliferationactivity assay showed that the overexpression of BNIPL-1 suppresses Hep3B cell growth in *** expression profiles of 588 known genes from BNIPL-1-transfected Hep3B-Tet-on and vectorcontrol cells were determined using the Atlas human cDNA expression *** genes were differentiallyexpressed between these two cell lines,among which seven genes were up-regulated and eight genes weredown-regulated by ***,the differential expression result was confirmed by *** these differentially expressed genes,p16INK4,IL-12,TRAIL and the lympbotoxin β geneinvolved in growth suppression or cell apoptosis were up-regulated,and PTEN involved in cell proliferationwas down-regulated by *** results suggest that BNIPL-1 might inhibit cell growth though cellcycle arrest and/or apoptotic cell death pathway（s）.

关键词： BNIPL-1 Tet-on Atlas human cDNA array apoptosis

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

爆炸作用下建筑结构高效毁伤评估方法

引用

爆炸与冲击 2024年

作者：吕晋贤吴昊卢永刚陈德 1.同济大学土木工程学院 2.中国工程物理研究院总体工程研究所同济大学土木工程学院中国工程物理研究院总体工程研究所

爆炸作用下建筑结构毁伤等级的高效预测有助于优化战前火力部署，评估战后毁伤效果，同时也可为重要建筑目标防护设计提供指导。然而，受限于建筑结构目标特性多样、体量庞大、内部系统庞杂以及验证困难等特点，尚缺乏高效的毁伤评估方... 详细信息

爆炸作用下建筑结构毁伤等级的高效预测有助于优化战前火力部署，评估战后毁伤效果，同时也可为重要建筑目标防护设计提供指导。然而，受限于建筑结构目标特性多样、体量庞大、内部系统庞杂以及验证困难等特点，尚缺乏高效的毁伤评估方法。借助高精度数值仿真分析，提出了综合考虑爆炸荷载、构件、房间和整体毁伤等级的建筑结构毁伤评估方法。首先，提出了爆炸作用下典型地面建筑，即含填充墙钢筋混凝土（RC）框架结构，损伤破坏和倒塌的高精度数值仿真分析方法，并得到RC结构爆炸试验和倒塌事故，以及砌体墙爆炸试验的充分验证。其次，开展了典型三层原型RC框架结构在不同爆炸当量（25～200kg TNT）作用下的内爆炸数值仿真，定量分析了爆炸冲击波在建筑结构内部的传播、结构损伤破坏和墙体飞散等。进一步给出了爆炸作用下建筑结构的高效毁伤评估流程，即：基于镜像爆源结合非线性叠加原理确定内爆炸荷载；基于等效单自由度方法评估梁、板、柱及墙体构件的毁伤等级；引入构件重要性系数加权确定房间毁伤等级；考虑房间功能及位置重要性评估整体结构的毁伤等级。最后，基于上述爆炸工况，通过对比高精度数值仿真分析与毁伤评估方法的预测结果，表明：两者得到的典型RC框架结构的整体毁伤等级一致，即在25kg、100kg和200kg TNT爆炸下分别呈现轻度、中度和重度毁伤，提出方法可缩短99%以上的计算耗时，兼具可靠性与时效性。

关键词：爆炸荷载建筑结构毁伤评估损伤破坏

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

余热锅炉入口管箱底面沉积特性研究

引用

化工学报 2024年

作者：赵浩然黄嗣罗林梅 1.西安交通大学能源与动力工程学院 2.茂名重力石化装备股份公司西安交通大学能源与动力工程学院茂名重力石化装备股份公司

采用计算流体力学软件，基于Euler-Lagrange方法，对一种余热锅炉入口管箱底面沉积特性进行数值模拟，详细研究了不同质量流量(0.002～0.010 kg·s-1)、入口速度(1.5～3.5 m·s-1)以及颗粒直径（20～60μm）对于底面沉积特性的影响规律... 详细信息

采用计算流体力学软件，基于Euler-Lagrange方法，对一种余热锅炉入口管箱底面沉积特性进行数值模拟，详细研究了不同质量流量(0.002～0.010 kg·s-1)、入口速度(1.5～3.5 m·s-1)以及颗粒直径（20～60μm）对于底面沉积特性的影响规律。模拟的气固两相速度与实验数据吻合较好，验证了模型的适用性。模拟结果表明：（1）当颗粒直径不变时，为了改善底面沉积情况，应当尽量减小入口速度和颗粒质量流量。（2）当颗粒质量流量不变时，若颗粒直径小于40μm，较小的入口速度能够较好地改善底面沉积情况；若颗粒直径大于40μm，增大入口速度能够显著改善底面沉积情况；若颗粒直径为40μm，入口速度的变化几乎不能改变底面沉积速率。（3）当入口速度不变时，为了改善底面沉积情况，应当尽量减小颗粒直径和颗粒质量流量。

关键词：气固两相流沉积数值模拟余热锅炉计算流体力学

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Comparative proteomic analysis of human osteosarcoma and SV40-im-mortalized normal osteoblastic cell lines

引用

Acta Pharmacologica Sinica 2007年第6期28卷 850-858页

作者： Qian-chen GUO~2,Jing-nan SHEN~(2,5),Song JIN~2,Jin WANG~2,Gang HUANG~2,Long-juan ZHANG~3,Jun-qiang YIN~2,Chang-yeZOU~2,Ming-tao LI~4~2 Department of Orthopedic Surgery,~3 Laboratory Center of Surgery and ~4 proteomic Center,Sun Yat-sen University,Guangzhou 510080,China Department of Orthopedic Surgery Sun Yat-sen University Guangzhou China Laboratory Center of Surgery Sun Yat-sen University Guangzhou China Proteomic Center Sun Yat-sen University Guangzhou China

Aim:Comparative proteomics provide a powerful approach in screening for alter-ations in protein levels and post-translational modifications that are associatedwith *** the present study,we aimed to identify candidate biomarkers todistinguish osteosarcoma (OS) cells from normal osteoblastic ***:Weemployed 3OS cell lines (U2OS,IOR/OS9,and SaOS-2),and used the SV40-immor-talized normal osteoblastic cell line (hFOB 1.19) as the *** differentialprotein levels in OS and osteoblastic cells were identified using 2-D gel electro-phoresis followed by matrix-assisted laser desorption/ionization-time of flightmass spectrometry *** proteins of interest,the levels of which weresignificantly increased in OS cells,were further characterized by Western ***:Twenty-six proteins were identified,the expression level ofwhich was either significantly increased or decreased in the OS cells as comparedto the control *** expression level of the activator of 90 kDa shock proteinATPase homolog 1 (AHA1),was enhanced 12.4-,24.1-,and 23.8-fold in SaOS-2,IOR/OS9,and U2OS cells,respectively,and the level of the stomatin-like protein 2(SLP-2) was increased by 10.4-and 7.8-fold in IOR/OS9 and U2OS cells,respectively,as compared to normal osteoblastic *** observations were confirmed byWestern blot ***:A differential proteomic analysis was suc-cessfully used to identify AHA 1 and SLP-2 that were significantly overproducedin OS cells as compared to normal osteoblastic cells,suggesting that those pro-teins among others may be effective biomarker candidates for the identification ofOS cells.

关键词： AHA1 differentially-produced protein osteosarcoma proteome SLP-2

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A prototype RF power source for CSNS/RCS

引用

Science China(Physics,Mechanics & Astronomy) 2011年第S2期54卷 218-221页

作者： WeiLing Huang (1) Hong Sun (1) HaiQi Zhao (2) Yu Liu (2) YanDong Li (2) ZhiGuang Qiao (2) Xiao Li (1) ChunLin Zhang (1) 1. Accelerator Center Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China2. 23rd Institute of China Aerospace Science and Industry Corporation Beijing 100854 China

A prototype RF power source has been built to supply high RF power to a ferrite-loaded cavity, which is a part of R&D of the Rapid Cycling Synchrotron of China Spallation Neutron Source (CSNS/RCS). A direct fast RF feedback amplifier, a 4:1 impedance transformer and auto tuning grid were locally located to compensate the heavy beam loading of CSNS/RCS. Design and commissioning of the RF power source is discussed here, also with some advice on system improvement.

关键词： RF power source synchrotron beam loading RF feedback amplifier

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

IL-1beta sensitizes rat intervertebral disc cells to Fas ligand mediated apoptosis in vitro

引用

Acta Pharmacologica Sinica 2007年第10期28卷 1671-1676页

作者： Li-yang CUI~2,Shang-li LIU~(2,4),Yue DING~2,Dong-sheng HUANG~2,Ruo-fan MA~2,Wei-guo HUANG~2,Bao-shan HU~2,Qiu-hui PAN~3~2 Department of Orthopaedic Surgery and ~3 Department of Lin Bai-Xin Medical Research Center,the Second Affiliated Hospital of Sun Yat-SenUniversity Guangzhou 510120,China Department of Orthopaedic Surgery the Second Affiliated Hospital of Sun Yat-Sen University Guangzhou China Department of Lin Bai-Xin Medical Research Center the Second Affiliated Hospital of Sun Yat-Sen University Guangzhou China

Aim:To determine the apoptotic effect of recombinant rat Fas Ligand on rat inter-vertebral disc cells pre-treated with IL-1beta in vitro,and the expression of Fas incultured rat intervertebral disc ***:Cells were isolated from the innerannulus fibrosus and transition zones of lumbar discs from Sprague-Dawley *** cells were grown in monolayer and divided in 5 treatment ***-1 beta (10ng/mL),FasL (5,20 ng/mL) with/without IL-1beta (10 ng/mL) pre-treatment wasrespectively added in Dulbecco’s modified Eagle’s medium and Ham’s F-12 me-dium with 1% fetal bovine *** 32 h,the cells were stained with annexin V-FITC and propidium iodide to evaluate apoptosis using flow cytometry and toanalysis transcription of Fas using ***:Compared with controlgroup,FasL (20 ng/mL),IL-1β(10 ng/mL)+FasL (5 ng/mL),and IL-1β(10 ng/mL)+FasL (20 ng/mL) induced significant apoptosis of the disc cells (P0.05).IL-1β(10 ng/mL) enhanced the apoptosis-inducing effects of FasL (5 ng/mL) and FasL (20 ng/mL) in disc *** genetranscription in all groups and Fas expression in the 5 treatment groups wereapproximately 1.2-2.1-fold greater than control group (respectively,P<0.05).Additionally,Fas expression in FasL with IL-1β pre-treatment groups were signifi-cantly up-regulated than in FasL groups (P<0.01).Conclusion:The results of thisstudy showed disc cells pre-treated with IL-lbeta increased apoptotic rate inresponse to FasL in vitro and provided insights to understand Fas/FasL system-mediated apoptosis in disc cells which would be enhanced due to inflammationfactor in degenerative disc.

关键词： intervertebral disc cell apoptosis Fas ligand flow cytometry interleukin-1 beta

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Proton and neutron alignments of the K~π=5/2^- band in ^(79)Kr

引用

Nuclear Science and Techniques 2000年第1期11卷 27-33页

作者： PAN Qiang-Yan1 De Poll M2 De Angelis G2 , Fahlander C2 Bazzacco D2 Farnea E2 Napoli D R2 Spolaore P2 (1Institute of Modeal Physics, the Chinese Academy of Sciences, Lanzhou 730000 2.aboratori Nazionali de Legnaro, INFN, 35030 Legnaro (PD), Italia 中科院近代物理所甘肃兰州 730000 Laboratori Nazionali de Legnaro INFN 35030

An experimental study of high-spin states in 79Kr has been performed at the GASP array using the reaction 55Mn(30Si, αpn). Nine new states along with 10 new gamma ray transitions were found in the Kπ=5/2- band in 79Kr. Three band crossings, associated with the alignments of a πg9/2 pair and a πg9/2 pair and also a high-j 5h11/2 intrude orbital, were observed at rotational frequency h=0.50, 0.65 and 0.90 MeV, respectively. A cranked shell model analysis has been made to interpret the experimental results.

关键词：氪79 高自旋态原子核质子队列中子队列自旋轨道形态

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Construction and Activity Assay of the Activating Transcription Factor 3 Reporter Vector pATF/CRE-luc

引用

Acta Biochimica et Biophysica Sinica 2006年第1期38卷 58-62页

作者： Jun-Qing XU~(1,2),Jing-Lan DENG~1,You-Sheng WU~2,Han-Yan FU~2,Rui-Hua WANG~2,Jian ZHANG~2, Fan LU~(2*),and Zhong-Liang ZHAO~(2*) ~1 Department of Naclear Medicine,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China ~2 Department of Biochemistry and Molecular Biology,Fourth Military Medical University,Xi'an 710032,China Department of Nuclear Medicine Xijing Hospital Fourth Military Medical UniversityXi'an 710032 China Department of Biochemistry and Molecular Biology Fourth Military Medical UniversityXi'an 710032 China

Activating transcription factor 3 (ATF3),a member of the activating transcription factor/cAMPresponsive element binding protein (ATF/CREB) family of transcription factors,is induced by manyphysiological *** investigate the activity of ATF/CREB in cells with physiological stresses,we developeda practical reporter vector,the plasmid pATF/CRE-luc,bearing activating transcription factor/cAMP responsiveelement (ATF/CRE) binding *** plasmid was constructed by inserting three repeats of the ATF/CREbinding element into the plasmid pG51uc,replacing the GAL-4 binding *** plasmids pACT/ATF3 andpATF/CRE-luc were transfected into HeLa and NIH3T3 cells,respectively,and the results showed that theexpression of luciferase .was increased in a dose-dependent manner on plasmid pACT/*** datasuggested that the plasmid pATF/CRE-luc could be used as a sensitive and convenient reporter system ofATF3 activity.

关键词： ATF/CRE luciferase gene pG51uc reporter system

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Overexpression of coxsackie and adenovirus receptor inhibit growth of human bladder cancer cell in vitro and in vivo

引用

Acta Pharmacologica Sinica 2007年第6期28卷 895-900页

作者： Lin-lin ZHANG~2,Da-lin HE~(2,3,4),Xiang LI~2,Lei LI~2,Guo-dong ZHU~2,Dong ZHANG~2,Xin-yang WANG~2~2 Institute of Urology,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China ~3 Institute of Oncology,Key Laboratory of Environ-ment and Genes Related to Diseases of the Ministry of Education,Xi'an Jiaotong University,Xi'an 710061,China Institute of Urology First Hospital of Xi'an Jiaotong University Xi'an China Institute of Oncology Key Laboratory of Environment and Genes Related to Diseases of the Ministry of Education Xi'an Jiaotong University Xi'an China

Aim:To study the effect of the overexpression of coxsackie and the adenovirusreceptor (CAR) on the growth of the human bladder cancer cell in vitro and ***:A retroviral vector pLXSN-CAR expressing CAR was constructedand confirmed by restriction enzyme *** pLXSN-CAR vector and con-trol vector pLXSN were transfected into the PT67 packaging cell line to generateretrovirus with high *** CAR-negative T24 cell was infected with the pLXSN-CAR and the pLXSN retrovirus,*** positive clone cells wereselected with G418 for 2 *** expression level of the CAR protein wasdetected by Western blot assay.T24 cell growth in vitro was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ***-age-independent growth was measured by soft-agar colony formation *** cell growth was determined by a nude mice xenograft ***:ThepLXSN-CAR vector containing full-length CAR cDNA was *** blot analysis showed that a 46 kDa specific band wasfound in pLXSN-CA-transfected T24 *** assay identified the growthinhibition of T24/pLXSN-CAR *** cell colony forming ability of T24/pLXSN-CAR cells was significantly lower than that of T24/pLXSN and parental T24 *** was a reduction in the tumor size in the T24/pLXSN-CAR group as com-pared with that of the T24/pLXSN group and parental T24 ***:The overexpression of CAR in T24 bladder cancer cells can inhibit cell growthboth in vitro and in vivo.

关键词： coxsackie and the adenovirus receptor bladder cancer cell growth nude mice

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：