Overexpression of coxsackie and adenovirus receptor inhibit growth of human bladder cancer cell in vitro and in vivo

作     者：Lin-lin ZHANG~2,Da-lin HE~(2,3,4),Xiang LI~2,Lei LI~2,Guo-dong ZHU~2,Dong ZHANG~2,Xin-yang WANG~2~2 Institute of Urology,First Hospital of Xi'an Jiaotong University,Xi'an 710061,China ~3 Institute of Oncology,Key Laboratory of Environ-ment and Genes Related to Diseases of the Ministry of Education,Xi'an Jiaotong University,Xi'an 710061,China 

作者机构：Institute of Urology First Hospital of Xi'an Jiaotong University Xi'an China Institute of Oncology Key Laboratory of Environment and Genes Related to Diseases of the Ministry of Education Xi'an Jiaotong University Xi'an China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2007年第28卷第6期

页      面：895-900页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Research Funds of Ministry of Health for Priority Clinical Programs China(№ 20012029) 

主　　题：coxsackie and the adenovirus receptor bladder cancer cell growth nude mice 

摘      要：Aim:To study the effect of the overexpression of coxsackie and the adenovirusreceptor (CAR) on the growth of the human bladder cancer cell in vitro and ***:A retroviral vector pLXSN-CAR expressing CAR was constructedand confirmed by restriction enzyme *** pLXSN-CAR vector and con-trol vector pLXSN were transfected into the PT67 packaging cell line to generateretrovirus with high *** CAR-negative T24 cell was infected with the pLXSN-CAR and the pLXSN retrovirus,*** positive clone cells wereselected with G418 for 2 *** expression level of the CAR protein wasdetected by Western blot assay.T24 cell growth in vitro was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) ***-age-independent growth was measured by soft-agar colony formation *** cell growth was determined by a nude mice xenograft ***:ThepLXSN-CAR vector containing full-length CAR cDNA was *** blot analysis showed that a 46 kDa specific band wasfound in pLXSN-CA-transfected T24 *** assay identified the growthinhibition of T24/pLXSN-CAR *** cell colony forming ability of T24/pLXSN-CAR cells was significantly lower than that of T24/pLXSN and parental T24 *** was a reduction in the tumor size in the T24/pLXSN-CAR group as com-pared with that of the T24/pLXSN group and parental T24 ***:The overexpression of CAR in T24 bladder cancer cells can inhibit cell growthboth in vitro and in vivo.