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Bio-regeneration of p-complexation desulfurization adsorbents

Science china Chemistry 2005年第6期48卷 538-544页

作者： LI Wangliang1,2. XING Jianmin1, XIONG Xiaochao1,2. SHAN Guobin1,2.& LIU Huizhou1 1. Laboratory of Separation Science and Engineering, Institute of Process Engineering, chinese academy of sciences, Beijing 100080, china 2. Graduate School of the chinese academy of sciences,Beijing 100039, china 1. Laboratory of Separation Science and Engineering Institute of Process Engineering Chinese Academy of Sciences 100080 Beijing China 2. Graduate School of the chinese academy of sciences 100039 Beijing China

The coupling of adsorption desulfurization and biodesulfurization is a new approach to produce clean fuels. Sulfur compounds are firstly adsorbed on adsorbents, and then the ad-sorbents are regenerated by microbial conversion. p-Complexation adsorbent, Cu(I)-Y, was ob-tained by ion exchanging Y-type zeolite with Cu2. and then by auto-reduction in helium at 450℃ for 3 h. Dibenzothiophene (DBT) was used as a model compound. The effects of cell concentra-tion, volume of oil phase, the ratio of aqueous phase to adsorbent on DBT desorption by a bac-terium were studied. The amounts of DBT desorbed and 2.HBP produced can be apparently increased with addition of n-octane. BDS activity can be improved by increasing cell concentra-tion and the ratio of water-to-adsorbent. 89% of DBT desorbed from the adsorbents can be converted to 2.HBP within 6 h and almost 100% within 2. h, when the volume ratio of oil-to-water was 1/5 mL/mL, the cell concentration was 60 g·L?1, and the ratio of adsorbent-to-oil was 0.03 g·mL?1. The amount of 2.HBP produced was strongly dependent on the volume ratio of oil-to- water, cell concentration and amount of adsorbent. Adsorption capacity of the regenerated ad-sorbent is 95% that of the fresh one after being desorbed with Pseudomonas delafieldii R-8, washed with n-octane, dried at 100℃ for 2. h and auto-reduced in He.

关键词： adsorption desulfurization p-complexation adsorbents bio-regeneration Pseudomonas delafieldii desorption.

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^(40)Ar/^(39)Ar dating of shear deformation of the Xianshuihe fault zone in west Sichuan and its tectonic significance

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Science china Earth sciences 2004年第9期47卷 794-803页

作者： ZHANG Yueqiao1, CHEN Wen2. 3 & YANG Nong1 1. Institute of Geomechanics, chinese {2. of Geological {2., Beijing 100081, china 2. Institute of Geology, chinese {2. of Geological {2., Beijing 100037, china 3. Institute of Geology and Geophysics, chinese academy of sciences, Beijing 10002., china Institute of Geomechanics Chinese Academy of Geological Sciences Beijing 100081 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

Based on field geological survey, structural measurements and classical Ar/Ar dating of mica, biotite and K-feldspar, we obtain cooling ages for Miocene left-lateral shear along the Xianshuihe fault zone. The results document two thermal events during the sinistral shear. The early event (12.10 Ma) corresponds to rapid cooling of the Zheduoshan granitic massif from above 700℃ to below 350℃. The late event (5-3.5 Ma) corresponds to cooling of granites intruded along the eastern side of the fault zone. These dating results provide important thermochronological constraint on the timing of late Cenozoic eastward extrusion of the Chuan-Dian Block in the SE Tibetan margin.

关键词： Xianshuihe fault zone, Zheduoshan granitic massif, sinistral shear deformation, 40Ar/39Ar dating, late Cenozoic.

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The deep thermal characteristic of continental margin of the northern South china Sea

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chinese Science Bulletin 2000年第18期45卷 1717-1722页

作者： ZHANG Jian & WANG Jiyang1. Graduate School, University of Science and Technology of china, chinese academy of sciences, Beijing 100039, china 2. Institute of Geology, chinese academy of sciences, Beijing 10002., china Graduate School University of Science and Technology of China Chinese Academy of Sciences Beijing China Institute of Geology Chinese Academy of Sciences Beijing China

Heat flow plays an important role in the study ot thermal structure and thermal evolutionof continental margin of the northern South china Sea. The analysis of heat flow value shows that margin heat flow in the northern South china Sea is relatively high setting, but the percentage of crustal heat flow is lower than 35% in terrestrial heat flow. The terrestrial heat flow exhibited a current of rise from the Northern Continental Margin to the Southern Central Basin. However, the proportion of crustal heat flow in terrestrial heat flow slowly dropped down in the same direction. It is suggested that the main factor causing high heat flow setting is the moving up of hot material from asthenosphere.

关键词： margin of northern South china Sea terrestrial heat flow thermal structures.

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CO_2.background concentration in the atmosphere over the chinese mainland

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chinese Science Bulletin 2002年第14期47卷 1217-1220页

作者： WANG Gengchen, WEN Yupu, KONG Qinxin, REN Lixin & WANG MulinInstitute of Atmospheric Physics, chinese academy of sciences, Beijing, 10002., {2. {2. of Meteorological sciences, {2. Meteorological Administration, Beijing 100086, {2. Institute of Atmospheric Physics Chinese Academy of Sciences Beijing China academy of Meteorological sciences China Meteorological Administration Beijing China

Based on the long-term monitoring data on CO2.concentration, variation trend and characteristics of CO2.background concentration in the atmosphere over the {2. mainland are analyzed. Results show that the increasing trend of CO2.background concentration in the atmosphere over the {2. mainland has appeared during the period of 1991-2.00. The average annual CO2.growth increment is 1.59μL/L, and the average annual CO2.growth rate is 0.44%. Distinct seasonal variations of CO2.background concentration are observed, and the averaged amplitude of CO2.seasonal variations is 10.35μL/L. Regional variation characteristics of CO2.background concentration in the atmosphere and possible impact of human activities on these variations over the {2. mainland are discussed as well.

关键词： CO2 background concentration chinese mainland CO2 growth rate CO2 seasonal variation CO2 regional variation CO2 variation trend.

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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, chinese academy of sciences, Wuhan 430072. china 2. Graduate School of chinese academy of sciences, Beijing 100039, china) [a]State key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China China [b]Graduate School of chinese academy of sciences Beijing China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

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Comparison of dynamic changes in endogenous hormones and sugars between abnormal and normal Castanea mollissima

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Progress in Natural Science:Materials International 2008年第6期18卷 685-690页

作者： Tao Liua,b,Yunqian Hua,*,Xiaoxian Li a akunming Institute of Botany,chinese academy of sciences,kunming 6502.4,china bGraduate University of the chinese academy of sciences,Beijing 100049,china kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China Graduate University of the chinese academy of sciences Beijing 100049 China

To elucidate the possible functions of endogenous hormones in the flowering of chestnut,concentrations of four endogenous hor-mones[indole-3-acetic acid(IAA),gibberellic acid(GA),abscisic acid(ABA),zeatin riboside(ZR))and the soluble sugars content were measured in both normal and developmentally abnormal chinese chestnut(Castanea mollissima)during flowering and fruiting *** results showed that the contents of ZR,ABA,and GA exhibited a significant dierent pattern in normal trees from that in abnormal trees,while the contents of IAA and soluble sugars showed a similar change pattern between *** results suggest that quantitative changes in endogenous hormones may correspond to dierent flowering and fruiting mechanisms.

关键词： Castanea mollissima Endogenous hormones Soluble sugars

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Cloning and characterization of interferon stimulated genes Viperin and ISG15,and their promoters from snakehead Channa argus

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Progress in Natural Science:Materials International 2007年第12期17卷 1425-1435页

作者： Jia Weizhang1,2. Zhou Xiuxia1,2. Huang Rong1,2.and Guo Qionglin1(1. Institute of Hydrobiololgy, chinese academy of sciences, Wuhan 430072. china 2. Graduate University of chinese academy of sciences, Beijing 100039, china) Institute of Hydrobiololgy Chinese Academy of Sciences Wuhan 430072 China Graduate University of chinese academy of sciences Beijing 100039 China

By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352.amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and γ-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2.recognition sites. GAS is responsible for the γ-IFN mediated transcription. One conserved site for NF-κB was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-κB in accordance with the consensus sequence (GGGRNNYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5′ untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I∶C.

关键词： interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus).

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Structures and physical properties of rigid polyurethane foams with water as the sole blowing agent

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Science china Chemistry 2006年第4期49卷 363-370页

作者： LI Xiaobin1,2. CAO Hongbin1 & ZHANG Yi1 1. Institute of Process Engineering, chinese academy of sciences, Beijing 100080, {2. 2. Graduate School of the chinese academy of sciences, Beijing 100039, {2. 1. Institute of Process Engineering Chinese Academy of Sciences Beijing 100080 China 2. Graduate School of the chinese academy of sciences Beijing 100039 China

Rigid polyurethane foams blown by varying water level were prepared in this study. The structures and physical properties of rigid polyurethane foams blown by water were measured with FT-IR, universal testing machine (Instron3365), scanning electron microscope (SEM) and differential scanning calorimeter (DSC). The results show that polyurea and polybiuret were the typical charac- teristics, and the cream time and gel time were shorter for the fully water blown rigid foams than that for the fully cyclopentane blown foams. The density of foam samples decreased from 45.0 kg/m3 to 2..4 kg/m3 with the increase of water level from 3 pph to 7 pph. Compressive strength exhibited the similar behavior with density. The average cell size of foam samples ranged from 2.1 μm to 356 μm with the increase of water level from 3 pph to 7 pph, respectively. At the same time, poorer dimen- sional stability was encountered with the increase of average cell size due to fast diffusion rate of CO2.out of the foam. The results of thermal analysis show that the glass transition temperature (Tg) shifted to a higher temperature with the increase of isocyanate level when more water was used as chemical blowing agent.

关键词： polyether polyol, polyurethane rigid foam, compressive strength, dimensional stability, cell size, glass transition temperature.

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Explicit analytical solutions of the anisotropic Brinkman model for the natural convection in porous media(Ⅱ)

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Science china(Physics,Mechanics & Astronomy) 2005年第4期48卷 422-430页

作者： CAI Ruixian1, GOU Chenhua1, 2.& ZHANG Na1 1. Institute of Engineering Thermophysics, chinese academy of sciences, Beijing 100080, {2. 2. Graduate School, chinese academy of sciences, Beijing 100080, {2. 1. Institute of Engineering Thermophysics Chinese Academy of Sciences 100080 Beijing China 2. Graduate School Chinese Academy of Sciences 10080 Beijing China

Some algebraically explicit analytical solutions are derived for the anisotropic Brinkman model―an improved Darcy model―describing the natural convection in porous media. Besides their important theoretical meaning (for example, in analyzing the non-Darcy and anisotropic effects on the convection), such analytical solutions can be the benchmark solutions that can promote the development of computational heat and mass transfer. Some solutions considering the anisotropic effect of permeability have been given previously by the authors, and this paper gives solutions including the anisotropic effect of thermal conductivity and the effect of heat sources.

关键词： explicit analytical solution, porous medium, natural convection, Brinkman model, anisotropic.

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The Chemical Classification of the AGB Star IRAS 17515-2.07

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chinese Journal of Astronomy and Astrophysics 2003年第6期3卷 551-554页

作者： Pei-Sheng Chen and Pin Zhang1National Astronomical Observatories, Yunnan Observatory, chinese academy of sciences, kunming 650011 {2.osmic Ray Institute, Yunnan University, {{2. 650091 Cosmic Ray Institute Yunnan University Kunming 650091

The chemical classification of IRAS 17515-2.07 has been debated for a longtime. Up to now there are two contenders, oxygen-rich or carbon-rich. We believe that IRAS17515-2.07 is an oxygen-rich source: because (ⅰ) it shows the silicate self-absorbed emission; (ⅱ)in the near infrared-IRAS diagram it is located in the oxygen-rich object region and (ⅲ)particularly, it has detected SiO maser emission.

关键词： stars: AGB stars: chemical classification stars: maser infrared: stars

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