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Comparative study on using carbon or nitrogen limited medium to culture white rot fungi for reactive brilliant red dye K-2BP decolotization under non-sterile conditions

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Science China Chemistry 2007年第5期50卷 718-724页

作者： GAO DaWen1.2, WEN XiangHua1.& QIAN Yi1.1.State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environment Science and Engi- neering, Tsinghua University, Beijing 1.0084, China 2 School of Forestry, Northeast Forestry University, Harbin 1.0040, China State Key Joint Laboratory of Environmental Simulation and PollutionControl Department of Environment Science and EngineeringTsinghua UniversityBeijing100084China School of Forestry Northeast Forestry UniversityHarbin 150040China

In order to explore ways for the application of white rot fungus in dye effluent treatment under non-sterile conditions, experiment on decolorization of reactive brilliant red was carried out, employing nitrogen-limited and carbon-limited medium with C/N ratio of 56/2.2 and 28/44 (in mmol/L), respectively. The results showed that the decolorization rate reached 92% while culturing white rot fungus with ni- trogen-limited medium; however, the decolorization process ended in carbon-limited medium (n(C)/n(N) = 28/44) because of bacterial contamination. In addition, pH rose up to 9.31.after 4 d of decolorization, which was caused by bacterial contamination in the carbon-limited system. Therefore, it is concluded that nitrogen-limited medium can inhibit bacterial growth to some extent while carbon-limited medium is more easily contaminated by bacteria. Nitrogen-limited medium is more suitable in culture of white rot fungus for decolorization of reactive dye. Medium with the ability of inhibiting yeast growth should be developed by adjusting other components of nitrogen-limited medium.

关键词： white rot fungus, Phanerochaete chrysosporium, non-sterile, decolorization, dye

Nitrogen Runoff and Leaching Losses During Rice-Wheat Rotations in Taihu Lake Region,China

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Pedosphere 2007年第4期17卷 445-456页

作者： TIAN Yu-Hua YIN Bin YANG Lin-Zhang YIN Shi-Xue ZHU Zhao-Liang State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences 71 East Beijing Road Nanjing 210008 （China）. Graduate College of the Chinese Academy of Sciences Beijing 100049 （China） College of Environmental Science and Engineering Yangzhou University Yangzhou 225009 （China）

Although nitrogen （N） loss through runoff and leaching from croplands is suspected to contribute to the deterioration of surrounding water systems, there is no conclusive evidence for paddy soils to prove this hypothesis. In this study, field plot experiments were conducted to investigate N losses through runoff and leaching for two consecutive years with 3 N fertilization rates in rice （Oryza sativa L.）-wheat （Triticum aestivum L.） rotations in the Taihu Lake region, China. A water collection system was designed to collect runoff and leachates for both the rice and wheat seasons. Results showed that dissolved N （DN）, rather than particulate N （PN）, was the main form of N loss by runoff. The NO3^--N concentration in runoff was between 0.1.and 43.7 mg L^-1. whereas the NH4^＋-N concentration ranged from below detection limit to 8.5 mg L^-1. Total N （TN） loads by runoff were 1.0-1..9 and 5.2-38.6 kg ha^-1.during rice and wheat seasons, respectively, and the main loss occurred at the early growing stage of the crops. Nitrogen concentrations in leachates during the rice seasons were below 1.0 mg L^-1.and independent of the N application rate, whereas those during the wheat season increased to 8.2 mg L^-1.and were affected by the fertilizer rate. Annual losses of TN through runoff and leaching were 1..7-48.1.kg ha^-1.from the rice-wheat cropping system, accounting for 5.6%-8.3% of the total applied N. It was concluded that reduction in the N fertilization rate, especially when the crop was small in biomass, could lower the N pollution potential for water systems.

关键词： leaching N fertilization rate N loss paddy soil runoff

A priori error analysis of a discontinuous Galerkin approximation for a kind of compressible miscible displacement problems

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Science China Mathematics 2010年第10期53卷 2679-2696页

作者： YANG JiMing1.& CHEN YanPing2 1.ollege of Science,Hunan Institute of Engineering,Xiangtan 41.1.4,China 2School of Mathematical Sciences,South China Normal University,Guangzhou 51.631.China 1. College of Science Hunan Institute of Engineering Xiangtan 411104 China2. School of Mathematical Sciences South China Normal University Guangzhou 510631 China

A kind of compressible miscible displacement problems which include molecular diffusion and dispersion in porous media are investigated.A symmetric interior penalty discontinuous Galerkin (SIPG) method is applied to the coupled system of flow and *** the induction hypotheses instead of the cut-off operator and the interpolation projection properties,a priori hp error estimates are *** error bounds in L2(H1. norm for concentration and in L∞(L2) norm for velocity are optimal in h and suboptimal in p with a loss of power 1.2.

关键词： a priori error discontinuous Galerkin compressible miscible displacement coupled system

Provable Efficient Certificateless Group Key Exchange Protocol

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Wuhan University Journal of Natural Sciences 2007年第1期12卷 41-45页

作者： CAO Chunjie MA Jianfeng MOON Sangjae Key Laboratory of Computer Networks and InformationSecurity Ministry of Education Xidian University Xi＇ an 710071Shaanxi China Mobile Network Security Technology Research Center Kyungpook National University Daeyu 702701 Korea

Certificateless public key cryptography （CL-PKC） avoids the inherent escrow of identity-based cryptography and does not require certificates to guarantee the authenticity of public keys. Based on CL-PKC, we present an efficient constant-round group key exchange protocol, which is provably secure under the intractability of computation Diffie-Hellman problem. Our protocol is a contributory key exchange with perfect forward secrecy and has only two communication rounds. So it is more efficient than other protocols. Moreover, our protocol provides a method to design efficient constant-round group key exchange protocols and most secret sharing schemes could be adopted to construct our protocol.

关键词： group key exchange certificateless public key cryptography provable security bilinear pairings

Induced pluripotent stem cell-related genes influence biological behavior and 5-fluorouracil sensitivity of colorectal cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第1期13卷 11-19页

作者： Zhong SHI1.Rui BAI1.Zhi-xuan FU1.Yong-liang ZHU2,Rong-fu WANG3,Shu ZHENG1.1.ancer Institute(Key Laboratory of Cancer Prevention and Intervention,China National Ministry of Education,Key Laboratory of Molecular Biology in Medical Sciences,Zhejiang Province,China),the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 31.009,China)(2Department of Gastroenterology,the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 31.009,China)(3Center for Cell and Gene Therapy,Department of Pathology and Immunology,Baylor College of Medicine,Houston,Texas 77030,USA) Cancer Institute Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education Zhejiang Province China.

Objective:We aimed to perform a preliminary study of the association between induced pluripotent stem cell(iPS)-related genes and biological behavior of human colorectal cancer(CRC) cells,and the potential for developing anti-cancer drugs targeting these ***:We used real-time reverse transcriptase polymerase chain reaction(RT-PCR) to evaluate the transcript levels of iPS-related genes NANOG,OCT4,SOX2,C-MYC and KLF4 in CRC cell lines and cancer stem cells(CSCs)-enriched tumor *** was knockdowned in CRC cell line SW620 by lentiviral transduction.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assays,plate colony formation,and a mouse xenograft model were used to evaluate alterations in biological behavior in NANOG-knockdown SW620 ***,mock-knockdown and NANOG-knockdown cells were treated with 5-fluorouracil(5-FU) and survival rate was measured by MTT assay to evaluate drug ***:A significant difference in the transcript levels of iPS-related genes between tumor spheres and their parental bulky cells was *** knockdown suppressed proliferation,colony formation,and in vivo tumorigenicity but increased the sensitivity to 5-FU of SW620 cells.5-FU treatment greatly inhibited the expression of the major stemness-associated genes NANOG,OCT4,and ***:These results collectively suggest an overlap between iPS-related genes and CSCs in *** a certain gene NANOG may truncate the aggressiveness of CRC cells.

关键词： Induced pluripotent stem cell Cancer stem cell Colorectal cancer NANOG 5-Fluorouracil

Reference ranges and age-related changes of peripheral blood lymphocyte subsets in Chinese healthy adults

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Science China(Life Sciences) 2009年第7期52卷 643-650页

作者： JIAO Yang1.QIU ZhiFeng1.XIE Jing1.LI DongJing2 & LI TaiSheng1.1.Department of Infectious Disease,Peking Union Medical College Hospital,Peking Union Medical College & Chinese Academy of Medical Sciences,Beijing,China 2 Department of Special Care Unit,Peking Union Medical College Hospital,Peking Union Medical College & Chinese Academy of Medical Sciences,Beijing,China Department of Infectious Disease Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing China Department of Special Care Unit Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing China

This study was performed to build region-specific reference ranges of peripheral blood lymphocyte subsets for Chinese healthy adults from the young to the elderly and analyze the trends of changes in lymphocyte subsets for evaluating the impact of age on the values.1.1.healthy adults aged 1.-86 were recruited based on the SENIEUR *** sets of reference ranges were finally built applicable for the healthy young(1.-44 years),middle-aged(45-64 years) and elder adults(≥65).Comparisons in parameters among the three cohorts showed that a statistically significant increase in CD1.CD56+ NK cell was observed between the middle-aged and elder cohorts,whereas for the majority of the parameters,a significant decline was observed between the young and the middle-aged *** results showed that inverse correlations were observed between the age and CD1.+ B,CD3+ T,CD3+CD4+ T,CD4+CD45RA+CD62L+ nave T cell and CD4+CD28+/CD4+,while the positive one was identified between the age and the NK *** significant changes of the most of immune parameters provided evidence for ***,T cell activation markers of CD8+CD38+ and CD8+HLA-DR+ showed reverse trends of association with age,which provides a clue for further researches on the mechanisms underlying the paradoxical clinical presentation of the elder patients.

关键词： lymphocyte subsets age reference range immunosenescence

BAF Complex Is Closely Related to and Interacts with NF1.CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 440-446页

作者： Li-Hui ZHAO~(1.2) Xue-Qing BA~1.Xiao-Guang WANG~3 Xiao-Juan ZHU~1.Li WANG~1.Xian-Lu ZENG~1. 1.Institute of Genetics and Cytology,Northeast Normal University,Changchun 1.0024,China 2 College of Agronomy,Jilin Agricultural University,Changchun 1.01.8,China 3 Department of Biology,Changchun Normal College,Changchun 1.0032,China InstituteofGeneticsandCytology NortheastNormalUniversityChangchun130024China

Brg-or hBrm-associated factor (BAF) complexes,a chromatin-remodeling complex family ofmammalian cells,facilitate transcriptional activity by remodeling nucleosome ***1.is the coresubunit of Brg-associated factor *** the present study,we investigated the spatial relationshipbetween Brg1.and nuclear factor 1.(NF1.CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptionalactivation in vivo by employing immuno-gold *** data showed that Brg1.was closely co-localizedwith NF1.CTF and RNAP Ⅱ in HeLa ***,the co-immunoprecipitation assay further revealedthat Brg1.can be isolated together with NF1.CTF and RNAP Ⅱ in the ConA-stimulated,but not the resting,T *** combined results suggested that BAF complexes can interact with NF1.CTF and RNAPⅡ,and this interaction is closely dependent on the activation of gene transcription.

关键词： BAF complex NF1.CTF RNAP Ⅱ gene transcription

腹板嵌入式钢-混凝土组合梁施工阶段受力性能研究

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土木工程学报 2009年第12期42卷 61-68页

作者：李国强李现辉张哲同济大学上海200092 同济大学土木工程防灾国家重点实验室上海200092

腹板嵌入式组合梁是一种经济适用的新型组合梁。它由无上翼缘的倒T形钢梁和混凝土翼板组合而成。其组合作用是靠倒T形钢梁腹板上部开槽,并嵌入到混凝土翼板中形成抗剪连接而实现的。由于钢梁无上翼缘,其侧向稳定性较差,不利于承受施工... 详细信息

腹板嵌入式组合梁是一种经济适用的新型组合梁。它由无上翼缘的倒T形钢梁和混凝土翼板组合而成。其组合作用是靠倒T形钢梁腹板上部开槽,并嵌入到混凝土翼板中形成抗剪连接而实现的。由于钢梁无上翼缘,其侧向稳定性较差,不利于承受施工阶段的荷载。对腹板嵌入式组合梁的施工阶段钢梁的整体稳定性能进行研究。首先结合倒T形钢梁的稳定性能,提出在下翼缘设置垫块的施工方法。其次考虑施工荷载的随机性进行单侧和双侧加载时的理论分析。钢梁双侧受力时,可由弹性稳定理论推导出整体稳定弯矩。钢梁单侧受力时,可以分解成对称受力和反对称受力的情况,分别求出其极限承载力,再按RANKINE法则得到最终承载力。为模拟实际受力状态,进行4根倒T形截面钢梁的试验和有限元分析,研究其受力与变形的关系,确定其极限承载力,并进行误差分析。最后进行试验、有限元分析和理论分析的结果比较,验证理论计算公式的合理性。

关键词：腹板嵌入式组合梁弯扭稳定 Rankine方法

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新型履带式全方位移动平台运动分析

浙江大学学报（工学版） 2018年第7期52卷 1345-1353,1397页

作者：杨怀彬张豫南黄涛房远张骞张杰陆军装甲兵学院兵器与控制系 63983部队

为了改善轮式全方位移动平台路面适应性、载重等方面的不足,基于全方位履带结构,采用与传统履带车辆类似的对称布局形式,设计新型的履带式全方位移动平台.根据平台布局特点,确定最优布局形式,建立运动学和动力学模型,分析平台运动的各... 详细信息

为了改善轮式全方位移动平台路面适应性、载重等方面的不足,基于全方位履带结构,采用与传统履带车辆类似的对称布局形式,设计新型的履带式全方位移动平台.根据平台布局特点,确定最优布局形式,建立运动学和动力学模型,分析平台运动的各向相异性.以中心转向运动为例进行对比分析可知,全方位平台转向滑动摩擦力矩和驱动力矩均减小.为了确保全方位平台具备和传统履带平台相同的转向能力,确定平台设计应满足的几何条件.根据设计原则,利用ADAMS软件建立平台虚拟样机,对平台纵向直行、横向直行、45°斜行、中心转向运动、E级不平路面和爬越楼梯进行仿真.仿真结果表明,该平台具备全方位移动性能和较好的路面适应性.

关键词：全方位移动平台履带转向运动辊轮偏置角各向相异性