The Arachidonic Acid Metabolome Reveals Elevation of Prostaglandin E2 Biosynthesis in Colorectal cancer
会议名称：《2024中国肿瘤标志物学术大会暨CACA整合肿瘤学高峰论坛暨第十七届肿瘤标志物青年科学家论坛暨中国肿瘤标志物产业创新大会》
会议日期：2024年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Colorectal cancer Arachidonic acid metabolomics Prostaglandin
摘 要：Arachidonic acid metabolites are a family of bioactive lipids derived from membrane phospholipids. They are involved in cancer progression, but arachidonic acid metabolite profiles and their related biosynthetic pathways remain uncertain in colorectal cancer(CRC). To compare the arachidonic acid metabolite profiles between CRC patients and healthy controls, quantification was performed using liquid chromatography-mass spectrometry-based analysis of serum and tissue samples. Metabolomics analysis delineated the distinct oxidized lipids in CRC patients and healthy controls. Prostaglandin(PGE2)-derived metabolites were increased, suggesting that the PGE2 biosynthetic pathway was upregulated in CRC. The qRT-PCR and immunohistochemistry analyses showed that expression levels of PGE2 synthases, the key protein of PGE2 biosynthesis,was upregulated in CRC, and positively correlated with CD68+ macrophage density and CRC development. Our study indicates that PGE2 biosynthetic pathway is associated with macrophage infiltration and progression of CRC tumors.