Ratiometric/Turn-on Fluorescent Probes with High Sensitivity and Specificity for Quantitative Detection of Superoxide Radical in Biological Systems
作者单位：Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and DiagnosticsSchool of PharmacyTianjin Medical University
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
关 键 词：Superoxide radical Fluorescent probe Self-immolative linker High Sensitivity
摘 要：Superoxide radical(O) plays vital roles in normal physiological processes and many diseases. Fluorescent technique has been established as the most widely used method for detection and imaging of Odue to high sensitivity and high temporal and spatial resolution. However, due to abundant antioxidants in biological systems and low reactivity of fluorescent probes with O, quantitative detection and imaging of Oat physiological levels is still challenging. To address this issue, herein, we report the synthesis of 19 Ofluorescent probes through conjugation of fluorophores, coumarin(CM), naphthalimide(NA) and 9 H-1,3-dichloro-7-hydroxy-9,9-dimethylacridine-2-one(DDAO) with trifluoromethanesulfonyl group via self-immolative linkers. Our results showed that 1) the introduction of the self-immolative linker increases the stability and specificity of the probes;2) increasing the electron-withdrawing property of the linker significantly enhances the reactivity of the corresponding probes with O;3) CM-and NA-based probes exhibit distinct emissions before and after reaction with O, enabling ratiometric detection of O. Our present study provides a new strategy for design of Oprobes and these probes also show great potential in quantitative detection and imaging of Oin various biological systems.