Microbial Transformation of Flavonoids by Isaria fumosorosea ACCC 37814
作者单位：The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural Sciences
会议名称：《中国生物工程学会第十三届学术年会暨2019年全国生物技术大会》
会议日期：2019年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学]
关 键 词：Isaria fumosorosea flavonoids microbial transformation methylglycosylation
摘 要：Glycosylation is an efficient strategy to modulate the solubility, stability, bioavailability and bioactivity of drug-like natural products. Biological methods, such as whole-cell biocatalyst, promise a simple but highly effective approach to glycosylate biologically active small molecules with remarkable regio-and stereo-selectivity. Herein, we use the entomopathogenic filamentous fungus Isaria fumosorosea ACCC 37814 to biotransform a panel of phenolic natural products, including flavonoids and anthraquinone, into their glycosides. Six new flavonoid(4-O-methyl)glucopyranosides are obtained and structurally characterized using high resolution mass and nuclear magnetic resonance spectroscopic techniques. These compounds further expand the structural diversity of flavonoid glycosides and may be used in biological study.