High-fat diet aggravates BDE47-inhibited testosterone production via DAX-1 of Leydig cells in rats
作者单位：State Key Lab of Reproductive MedicineInstitute of ToxicologyNanjing Medical University Key Lab of Modern Toxicology of Ministry of EducationSchool of Public HealthNanjing Medical University
会议名称：《江苏省遗传学会2016年学术年会》
会议日期：2016年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
关 键 词：High-fat diet 2,2’,4,4’-tetrabromodiphenyl ether DAX-1 insulin testosterone primary Leydig cells
摘 要：Growing evidence has revealed that a high-fat diet（HFD） could lead to disorders of glycolipid metabolism and insulin-resistant states,and a HFD has been associated with the inhibition of testicular steroidogenesis and spermatogenesis,that is closely related to the transmission of genetic *** previous study demonstrated that 2,2’,4,4’-tetrabromodiphenyl ether（BDE47） could increase the risk of diabetes in humans,reduce testosterone production in ***,whether a HFD affects BDE47-inhibited testosterone production by elevating insulin levels and inducing related pathways remains *** rats were treated with BDE47 combined with or without HFD by gavage for 12 weeks and body weight（bw） was quantified *** BDE47 content in liver and testes in rats were detected with a gas chromatograph-mass spectrometer（GC/MS） using BDE77 as an internal *** of serum biochemical parameters and testosterone were conducted by using standard kits and radioimmunoassay kits *** observation of tissues in rats were determined using an optical *** addition,an isolated Leydig cells model was performed to detect the testosterone production in *** synthesis related proteins were measured by immunoblotting assay,immunocytochemistry and immunohistochemistry *** rats treated with BDE47,a HFD significantly increased the BDE47 content of the liver and testis and increased the body weight gain and the relative weight of the adipose tissue;increased macrovesicular steatosis in the liver and the levels of triglycerides,fasting glucose and insulin;further aggravated the destruction of the seminiferous epithelium;lowered the level of testosterone,and resulting in fewer sperm in the epididymis,disrupted spermatogenesis and *** note,a HFD enhanced BDE47-induced DAX-1 expression and decreased the expression levels of testosterone synthesis related proteins,such as StAR and 3β-HSD in the testicular in