Lateral septum GABAergic neurons regulate active-period sleep and stress-induced insomnia
作者单位：Department of PharmacologySchool of Basic Medical SciencesFudan University
会议名称：《中国睡眠研究会第十届全国学术年会》
会议日期：2018年
关 键 词：Chemogenetics Insomnia Lateral septum Sleep
摘 要：Sleep architecture is often disturbed following a stressful event;nevertheless,the brain circuitry responsible for sleep perturbations by stress has been paid little attention.The lateral septum（LS） has been shown to have a key role in stress responses and involved in the regulation of cortical activity.However,the exact role of the LS on sleep modulation is not clear.In this study,neuronal activity in LS GABAergic neurons of freely behaving mice was recorded by fiber photometry technology,and chemogenetic and cell-selective lesion approaches were used to identify the essential role of the LS in regulating the sleep-wake behavior.Results showed that LS GABAergic neurons increased their population activity at arousal state.Then,chemogenetic activation of LS GABAergic neurons expressing hM3 Dq receptors significantly increased the non-rapid eye movement（NREM） sleep during the active period,whereas chemogenetic inhibition of LS GABAergic neurons led to a significant decrease of NREM sleep at inactive period,but not active period of mice.Moreover,selectivity lesions of LS GABAergic neurons significantly increased wakefulness during the active period and aggravated insomnia caused by a psychological stressor（cage exchange） at inactive period.Finally,dysfunction of the lateral septal nucleus significantly increased c-fos expression in the basal forebrain and lateral hypothalamus during the cage exchange.Conclusively,these findings indicate that the LS GABAergic neurons reveal a prominent contribution to regulate the active-period sleep and stress-induced insomnia.