The arousal effects of alarm pheromonesand their neural pathways involved
作者单位：Departments of NeuroscienceAnatomyHistologyand EmbryologyKey Laboratory of Preclinical Study for New Drugs of Gansu ProvinceSchool of Basic Medical SciencesLanzhou University
会议名称：《第五届中国西部睡眠医学大会暨重庆市心理卫生协会睡眠医学学术年会》
会议日期：2018年
基 金：partly supported by:81471347,81171254 and 81071076 from NSFC to Yi-Ping Hou and 31500853 to Yu-Feng Shao lzujbky-2015-167,277 and lzujbky-2017-135 from Fundamental Research Funds for the Central University to Yu-Feng Shao
关 键 词：alarm pheromones 2-sec-butyl-4,5-dihydrothiazole non-rapid eye movement sleep rapid eye movement sleep arousal Grueneberg ganglion vomeronasal organ locus coeruleus
摘 要：Objective:Alarm pheromones(APs) are important chemical communicating molecules which signal danger,injury,distress,or the presence of predators to *** is unclear as yet whether APs could wake up rodent during sleep period to avoid dangerousness,through a sufficient sleep is required for their ***:Free Moving Olfaction-Sleep Recording System(FMOSRS) was used to analyze sleepwake states in this ***(collected from mice with electrical stimulation) or their main component(2-sec-butyl-4,5-dihydrothiazole,SBT) were directly delivered to the mouse’s nasal cavity during the period of non-rapid eye movement(NREM) or rapid eye movement(REM) sleep determined by EEC/EMC and digital *** of axons of the Cr(u|¨)eneberg ganglion(CCAxo) or excision of the vomeronasal organ(VNOX) were used to identify the sensory organ of *** addition,microinjection of herpes simplex virus(HSV) was employed to reveal the neural pathways involved in APs-induced ***:(1) Both of APs and SBT deliveries immediately induced an arousal from NREM or REM sleep compared to air in *** SBT-induced awakening efficiency was higher than APs-induced.(2) GG neurons degenerated by GGAxo,and showed an attenuation of the arousal effects of SBT mainly in post-odor stimulate period.(3) VNOX blocked the arousal effects of SBT mainly in odor stimulate period.(4) HSV labeling neurons in the basal forebrain(BF) and locus coeruleus(LC) were found following olfactory bulb(OB) *** percentage of HSV neurons in the LC that also display staining for tyrosine hydroxylase(TH) were 75.70%± 3.20%,whereas,HSV neurons in the BF were not cholinergic ***:Nasal delivery of APs or SBT arouses mice from NREM and REM *** and VNO are sensory organs for detecting APs,because the arousal effects of SBT are blocked by GGAxo and ***,HSV injection into the OB markedly labeled LC neurons,most of them also express *** results suggest that APs ar