Overexpression of athaumatin-like protein gene fused with a prolinerich peptide enhanced disease resistance of Sclerotiniasclerotiorum and Botrytis cinerea in Arabidopsis
作者单位：The Key Laboratory of Biology and Genetic Improvement of Oil CropsThe Ministry of Agriculture of PRCOil Crops Research InstituteChinese Academy of Agriculture Sciences
会议名称：《2018全国植物生物学大会》
会议日期：2018年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学]
关 键 词：B.napus BnTLP-PRP1 S.sclerotiorum B.cinerea JA/ET pathway
摘 要：In plants,pathogenesisrelatedproteins(PRs) play vital role in defenseresponse of various biotic and abiotic *** studies have showed the antifungal activity of the PR-5 protein(thaumatin-like proteins,TLPs).However,the molecularbasis of TLPs for resistance to necrotrophic fungal pathogens,mchidmgSclewtiniasclerotiorum and Botrytiscinerea,is still ***,we cloned a novel TLP gene in ***,which fused a PRP domain in its C termini,named *** on the collinear analysis across the well-defined phylogenetic analysis,we deduced origin of this fusion *** expression of BnTLP-PRPl in Arabidopsis led to significant enhanced tolerance to *** and B.c/flerazinfections,while the T-DNA mutant of the endogenous AtTLP-PRPl geneshowed more susceptible to both *** interestingly,both the TLP and PRP domain could contribute to the disease ***,the expression of numerous marker genes involved in salicylic acid(SA) and jasmonic acid(JA)/ethylene(ET) signaling pathways were dramatically *** together,these results indicate that the BnTLP-PRPl,an ancient fusion gene,regulates resistance to fungal pathogens by governing both SA and JA/ET-mediated defense signaling in Arabidopsis.