Track His-tagged Proteins and Metalloprpteomes Inside Live Cells by New Fluorescent Probes
作者单位：Department of Chemistry The University of Hong Kong
会议名称：《第十届全国化学生物学学术会议》
会议日期：2017年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
基 金：supported by the Research Grants Council of Hong Kong(704612,703913,17304614,1730541P) Livzon Pharmaceutical Group the University of Hong Kong(for an emerging Strategic Research Theme(e-SRT)on Integrative Biology)
摘 要：正Fluorescence-based techniques are promising analytical tools in tracking protein localization and in facilitating the investigation on protein functions （1）. Small-molecular based fluorescent probes can be designed to selectively interact with small peptide-tag on target proteins to elucidate the their localizations and dynamic activities. One of the most widely applied genetic modification is to utilize the hexahistidine-tag system （His-tag） in