A spatial-resolved electrochemiluminescence aptasensor for protein detection in a double-check mode
作者单位：College of Chemistry and Chemical EngineeringXinyang Normal University
会议名称：《河南省化学会2018年学术年会》
会议日期：2018年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
关 键 词：Carcinoembryonic antigen Silver-polyamidoamine nanoparticles Resonance energy transfer CdS-C composites Luminol-gold nanoparticles
摘 要：A spatial-resolved electrochemiluminescence(ECL)aptasensing platform for sensitive protein detection was constructed in a double-check ***-C composites as cathodic ECL emitters and luminol-gold nanoparticles(L-Au NPs)as anodic ECL emitters were immobilized on working electrode 1(WE1)and working electrode 2(WE2)of the dual-disk glassy carbon electrodes(DDCE),*** aptasensing platform was designed by integrating Ag-polyamidoamine NCs-labeled biotin-aptamer(Ag-PAMAM-bio-Apt)with the ECL emitters on the *** in-situ consumption of co-reactants,*** in the ECL system by Ag-PAMAM NCs resulted in the great ECL quenching on both WE1 and *** resonance energy transfer between L-Au NPs and Ag-PAMAM NCs made the ECL on WE2 further *** the presence of carcinoembryonic antigen(CEA),portions of Ag-PAMAM-bio-Apt detached from the DDCE because of the competitive reaction of CEA and cDNA toward to Ag-PAMAM-bio-Apt,generating an enhancement of ECL *** strategy achieves the detection of CEA in a double-check mode with detection limits of 0.39 pg m L for the cathodic assay(WE1)and 0.20 pg m L for the anodic assay(WE2),***,the proposed method had been successfully applied to determine CEA in human serum sample with recoveries of80.0?110.0%,showing great potential in cancer diagnosis.