Quantitative proteomics reveals the central changes of wheat in response to powdery mildew
作者单位：College of AgronomyNorthwest A&F University China State Key Laboratory of Crop Stress Biology for Arid Areas
会议名称：《第六届全国小麦基因组学及分子育种大会》
会议日期：2015年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Wheat iTRAQ Quantitative proteomics Powdery mildew
摘 要：Powdery mildew(Pm),caused by Blumeriagraminis ***(Bgt),is one of the most important crop diseases,causing severe economic losses to wheat belts *** the present study,an iTRAQ-based quantitative proteomic technique was applied to understand the molecular mechanism involved in resistant wheat(line N9134)response to Bgt isolate *** found 394 proteins that show a significant difference,which systematically reveal the protein expressive characterization of stimulus responses to *** differentially expressed proteins(DEPs) included members of different plant defense categories and metabolic pathways,such as pathogenesis-related(PR)polypeptides,oxidative stress responsive proteins,components involved in primary metabolic *** addition,RNA sequencing(RNA-seq) data further demonstrated that these cluster genes were co-transcribed,and their mRNA abundance changes exhibited the similar trends,suggesting that the Bgt-induced proteins are partly correlated with the correspondent transcription *** enrichment analysis showed that phenylpropanoid biosynthesis,phenylalanine metabolism and photosynthesis-antenna proteins were the key pathways in response to Bgt *** 5 and the Gibbs Motif Sampler cluster 394 DEPs to eight conserved motifs,which shared leucine repeats and histidine site in the sequence ***,eight separate protein-protein interaction(PPI) networks were predicted from STRING *** summary,our findings reveal multiple levels of regulation under powdery mildew infection in *** large amounts of protein and transcript data in this study provide a powerful platform for further exploration of the molecular mechanism underlying the wheat response to Bgt.