Repopulated microglia are solely derived from the proliferation of residual microglia, not from de novo progenitor cells
作者单位：Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shanghai Center for Bioinformation Technology City University of Hong Kong School of Psychology Nanjing Normal University State Key Laboratory of Brain and Cognitive Sciences The University of Hong Kong Guangdong-Hong Kong-Macau Institute of CNS Regeneration Jinan University Department of Ophthalmology Keck School of Medicine University of Southern California School of Biomedical Sciences Li Ka Shing Faculty of Medicine The University of Hong Kong
会议名称：《中国神经科学学会第十二届全国学术会议》
会议日期：2017年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：supported by National Natural Science Foundation of China (31600839) (B.P.) Guangdong Innovative and Entrepreneurial Research Team Program (2013S046) (B.P.) Shenzhen Peacock Plan (B.P.)
关 键 词：Microglia repopulation CSF1R fate mapping parabiosis
摘 要：Studies found new-born microglia replenished the whole brain after selective elimination of microglia（99%） in adult mice. Immunohistochemical evidences suggested that repopulated microglia were differentiated from de novo progenitors expressing Nestin in the brain, which raised the possibility that the cross-lineage differentiation occurs in the mature brain. However, the origin of repopulating microglia has been hotly debated. In the present study, we investigated the origin of repopulating microglia by fate mapping. We first excluded that repopulated microglia were from blood cells. We then identified that repopulated microglia were not differentiated from Nestinpositive cells. Next we demonstrated all new-born microglia were derived from the proliferation of surviving microglia（0.90%）. Moreover, we confirmed that the whole brain transcriptome were not largely altered among stages of microglial elimination and repopulation. In summary, we concluded that de novo microglial progenitor cells were not existed in the adult brain.