High throughput sequencing reveals endophytic fungal communityin field-grown soybean of Huang-huai-hai region of China
作者单位：Plant Pathology DepartmentNanjing Agricultural University
会议名称：《中国植物病理学会2017年学术年会》
会议日期：2017年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Endophytic fungi Soybean ITS Plant pathogens Microbiome
摘 要：Plants depend upon beneficial interactions between roots and endophytic fungal for growth promotion,disease suppression,and stress *** this study,we analyzed the endophytic fungal community harbored in the root of soybean plants grown in field in Huang-huai-hai region,one of the main producing areas of soybeans in *** on ITS amplicon sequencing for soybean samples collected from Shandong,Jiangsu,and Anhui province,we found the majority of endophyte taxa were Ascomycota（87%）,followed by Basidiomycota（10%） and Zygomycota（1%）.The species composition and abundanceof endophytic fungi were significantly dependent on the geographic locality and the plant developmental *** deep sequencing results were confirmed by traditional methods for isolation of fungi from corresponding *** most frequendy isolatesin genus were Haematonectria（60%）,Phoma（10%）,Fusarium（8%）,Rhizoctonia（6）,Clonostachys（6）,Macrophomina（3）,and Ceratobasidium（2%）.Many phytopathogenic species associated with soybean disease were found;however,it remains to be determined whether these endophytes are latent pathogens or non-pathogenic forms that benefit the *** results have revealed the endophytic fungal community in field-grown soybean of Huang-huai-hai region,providing insight for further study of plant-endophyte interaction.