Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among Coenurus infecting animals and humans from different regions of China
作者单位：College of Veterinary Medicine Hunan Agricultural University
会议名称：《中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会》
会议日期：2016年
学科分类：090601[农学-基础兽医学] 1002[医学-临床医学] 09[农学] 0906[农学-兽医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：the program of local standard in Hunan province No.5026301-1112006
关 键 词：cox1 12S rRNA ITS T.Multiceps sequence analysis phylogenetic relationship
摘 要：Objectives Sequence variability in two mitochondrial DNA(mtDNA) regions, namely cox1 and 12 S rRNA, and ITS of rDNA in Coenurus from different geogr-aphical regions in China was examined to analyze the variation of three sequences. Methods A portion of the cox1(pcox1), ITS genes and the 12 S rRNA(p12S) were amplified respectively from individual sample by PCR. Representative amplicons were subjected to sequencing for estimating sequence variability. Results The sequences of pcox1, 12 S rRNA and ITS were 421, 617 bp and 1320 in length, severally. the level of pairwise sequence variation between individual haplotypes were concluded to be 0—1.6% for the pcox1, 0—0.9% for the 12 S rRNA, 0.3%—4.2% for ITS. while, the inter-specific sequence variation were dramatically higher,being 9.9—28.7%, 8.3—26.4%,36—72.34% for pcox1,12 S rRNA and ITS, respectively. Phylogenetic analyses based on the 12 S rRNA sequence data revealed that T. multiceps and T. hydatigena were more closely related to the other members of the Taenia genus, and parasites of genus Echinoco- ccus were sister to the genus Taenia. Conclusion we could make a decision that Coenurus cerebralis and Coenurus skrjabini were belonged to the larval development of Taenia *** findings demonstrated clearly the usefulness of mtDNA and rDNA sequences for population genetic studies of these cestodes of socio-economic importance.