Interaction of glycyrol with calcineurin A studied by spectroscopic methods and docking
作者单位：Department of Biochemistry and Molecular BiologyBeijing Normal UniversityBeijing Key Laboratory
会议名称：《中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议》
会议日期：2010年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
关 键 词：calcineurin glycyrol fluorescence spectroscopy circular dichroism docking
摘 要：We have shown previously that glycyrol has an inhibitory effect on the immune response in mice by reducing calcineurin ***,we investigated the interaction of glycyrol with calcineurin A(CNA,catalytic A subunit of calcineurin) by spectroscopic methods and *** analyzing the fluorescence emission spectra of CNA with various concentration of glycyrol at 4℃,the binding constant (KA) and the number of binding sites(n) were calculated. For calculating thermodynamic parameters of interaction, KA at 25℃was previously calculated to be 2.55(±0.17)×105 M-l(n=3,relative coefficient R0.99).Then AH and