The antiarteriosclerosis mechanisnis of Scallop skirt-glycosaminoglycan
会议名称：《第十五届国际药理学大会》
会议日期：2006年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
关 键 词：SS-GAG. arteriosclerosis macrophage VSMCs endothelial cells
摘 要：正 In order to investigate the antiarteriosclerosis mechanisms of scallop skirt-glycosaminoglycan（SS-GAG）, RT-PCR analysis, immunohistochemical staining, enzymatic method and flow cytometry so on were employed. SS-GAG can inhibit the formation of foam cells derived from macrophage（ U937） and vascular smooth muscles cells （ VSMCs）. and decrease the overexpression of some cytokines （TNFα, IL-6 and IL-8） and intracellular Ca2+ level. Furthermore, SS-GAG up-regulated the mRNA and protein expression of low density lipoprotein （LDL） receptors and scavenger receptor class B, type Ⅰ（SR-BI）, a well characterized high density lipoprotein （ HDL） receptor in macrophage （ RAW264.7） . It also down-regulated the overexpression of scavenger receptor CD36 induced by Ox-LDL lesion, restrained the oxidative modification of RAW264.7 to ***-GAG can modulate lipoprotein metabolic disorders and the expression of some lipoprotein receptors. At the same time. SS-GAG inhibited excessive proliferation of VSMCs and protected endothelial cells from oxidation damage. The above is mavbe the antiarteriosclerosis mechanisms of SS-GAG.