Studies on Identification of Wheat-Barley 2H Alien Substitution Lines
会议名称：《吉林省第六届生命科学大型学术报告会》
会议日期：2008年
关 键 词：wheat barley substitution line Genomic in situ hybridization (GISH) RFLP
摘 要：The genetic constitution of fifteen materials derived from the cross CS (Triticum aestivum ***. Chinese Spring)x Betzes (Hordeum vulgate ***) was analyzed,and six disomic alien substitution lines were screened by *** chromosome configurations in pollen mother cells at meiotic metaphase I(PMCs M I) of F from each disomic substitution line respectively crossed with double ditelocentric lines 2A,2B and 2D of Chinese Spring were observed,and a set of wheat-barley disomic alien substitution lines 2H(A),2H(B) and 2H(D) were *** RFLP analysis with the probe psr131 on the short arm of wheat homeologous group 2 combining with four restriction enzymes were carded *** results indicated that the probe psr131 could be used as molecular marker to tag the barley chromosome *** barley chromosome 2H had good genetic compensation ability for wheat chromosome 2B and 2D in vitality and other agronomic *** result of testing seed was that the wheat appearance starch quality had been changed from the half-farinaceous of CS to the half-cutin of substitution lines by transferring the barley chromosome 2H to wheat.