Role of 5-lipoxygenase in hippocampal neuron damage following global cerebral ischemia in rats
会议名称：《中国药理学会第十一次全国学术会议》
会议日期：2011年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
关 键 词：global cerebral ischemia hippocampus neuron 5-lipoxygenase zileuton
摘 要：OBJECTIVE To determine spatio-temporal properties of 5-lipoxygenase(5-LOX) expression and the effect of zileuton, a selective 5-LOX inhibitor,on the hippocampal neuron injury following global cerebral ischemia(GCI).METHODS GCI was induced by bilateral common carotid artery occlusion combined with hypotension in rats.5-LOX expression was detected by Western blot analyses and 5-LOX localization was visualized by immunohistochemistry and double immunoflurescence.The 5-LOX inhibitor zileuton(10,30,50 mg·kg) were orally administered.RESULTS The 5-LOX expression was significantly upregulated in the hippocampus 1,3 and 7 d(peaked at 3 d) after ischemia.In the hippocampal CA1 region,5-LOX protein was primarily localized in the neurons.The 5-LOX inhibitor zileuton(30,50 mg·kg) markedly reduced hippocampal CA1 neuron damage at 3 d after ischemia. Protective effect of zileuton(30 mg·kg,7 consecutive days) is still evident 14 d after the ischemia episode.CONCLUSION 5-LOX is involved in global cerebral ischemic damage,the 5-LOX selective inhibitor zileuton possesses neuroprotective effect on neuronal damage in the rat hippocampus following GCI.