Transcription factor RBP-J-mediated signaling represses the differentiation of neural stem cells into intermediate neural progenitors

作     者：Fang GAO~(1,2),Qi ZHANG~(1,2),Min-Hua ZHENG~1,Hui-Ling LIU~2,Yi-Yang HU~1,Ping ZHANG~1,Zheng-Ping ZHANG~1,Hong-Yan QIN~1,Lei FENG~1,Li WANG~1,Hua HAN~(1,2,*),Gong JU~(2,*) 1 Department of Medical Genetics and Developmental Biology,Fourth Military Medical University,Xi''an 710032, China 2 Institute of Neurosciences,Fourth Military Medical University,Xi''an 710032,China 

会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》

会议日期：2009年

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

关 键 词：neural stem cells intermediate neural progenitors notch RBP-J neuron glia 

摘      要：正Notch signaling is critical for multiple aspects of neurogenesis,but how it regulates the proliferation and differentiation of neural stem cells（NSCs） and intermediate neural progenitors（INPs） has not been well elucidated, especially in *** this study,we conditionally ablated the transcription factor RBP-J,which mediates signaling from all four mammalian Notch receptors,in the basal forebrain and ventral midbrain using the RBP-J-floxed mouse and a newly established Nestin-Cre *** found that at early stage of neurogenesis（E11.5）,the frequency of neurospheres increased significantly in the RBP-J-inactivated *** majority of the RBP-J deficient neurospheres were composed of INPs,suggesting precocious differentiation of NSCs into ***,neuronal differentiation was reduced in the same regions at E11.5,inconsistent with the precocious differentiation phenotype in most Notch-related *** late neurogenic stages（E17.5 and neonatal）,as expected from precociously exhausted NSC pool,neurosphere frequency and NSCs decreased in the RBP-J-ablated regions,accompanied by significant increases of both neurons and glial *** results indicate that the RBP-J-mediated signaling might inhibit the differentiation of NSCs into INPs and support the generation of certain early born neurons at early neurogenic stages.