Effect of daidzein on lactobacillus community change in piglet digesta during in vitro fermentation as analyzed by DGGE technique
作者单位：Wageningen Institute of Animal Sciences Wageningen University Wageningen Netherlands Wageningen Institute of Animal Sciences Wageningen University Wageningen Netherlands Laboratory of Microbiology Wageningen University Hesselink van Suchtelenweg 4 6703 CT Wageningen Netherlands
会议名称：《2003北京国际养猪研讨会暨展览会》
会议日期：2003年
关 键 词：Denaturing gradient gel electrophoresis （DGGE） lactobacillus daidzein dilution PCR digesta piglet
摘 要：正 The effect of daidzein on Lactobacillus community change during in vitro fermentation ofpiglet digesta from different gastrointestinal （GI） compartment obtained at different ages was investigated.Every three conventionally raised healthy piglets from the same litter were slaughtered at age of 7, 14, 21and 28 days respectively. On each day of slaughtering, the digesta from the same GI compartment （jejunum,ileum, caecum and rectum） was pooled. The pooled digesta from each compartment was used as inocula inthree in vitro fermentation treatments: （a） VanSoest medium with 0.5g of glucose and 50ppm of daidzein;（b） VanSoest medium with 0.5g of glucose;（c） VanSoest medium only.After 48 hours fermentation,