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Effects of daidzein on antioxidant capacity in weaned pigs and IPEC-J2cells

Animal Nutrition 2022年第4期11卷 48-59页

作者： Yanpin Li Xianren Jiang Long Cai Yanli Zhang Hongbiao Ding Jingdong Yin Xilong Li Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Our previous study found that soybean isoflavones in soybean meal play an important role in improving growth performance and antioxidant capacity in pigs.However,it is still unknown whether long-term supplementation with daidzein,an active molecule deglycosylated from daidzin,in a corn-soybean meal diet can enhance growth performance in pigs.Thus,in the present study,an animal trial was carried out to investigate the effects of dietary supplementation with daidzein on the growth performance and antioxidant capacity of pigs.A total of 80 weaned piglets(40 barrows and 40 females)were assigned to 4treatments with 5 pens per treatment and 4 piglets per pen and fed a diet supplemented with 0,25,50 and 100 mg/kg daidzein for a 72-day trial.In addition,porcine intestinal epithelial cells(IPEC-J2)were used as an in vitro model to explore the underlying antioxidant mechanisms of daidzein.IPEC-J2 cells were treated with 0.6 mM hydrogen peroxide(H_(2)O_(2))in the presence or absence of 40μM daidzein.The results showed that adding 50 mg/kg of daidzein to the diet significantly improved body weight on day72,average daily gain(ADG)during days 0 to 72 and plasma superoxide dismutase(SOD)activity on day42,and decreased malondialdehyde(MDA)content on day 14(Pdaidzein prevented the decrease in cell viability and CAT activity and the increase in intracellular ROS levels and MDA content caused by H_(2)O_(2)(Pdaidzein preserved the expression of Nrf2,CAT and occludin in H_(2)O_(2)-stimulated IPEC-J2 cells(Pdaidzein imp

关键词： daidzein Growth performance Antioxidant capacity IPEC-J2 Oxidative stress Pig

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Neuroprotective effects of daidzein on focal cerebral ischemia injury in rats

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Neural Regeneration Research 2015年第1期10卷 146-152页

作者： Adem Bozkurt Aras Mustafa Guven Tar?k Akman Adile Ozkan Halil Murat Sen Ugur Duz Y?ld?ray Kalkan Coskun Silan Murat Cosar Department of Neurosurgery Canakkale Onsekiz Mart University Department of Neurology Canakkale Onsekiz Mart University Department of Medical Biochemistry Ministry of HealthAdministration of Public Health Deparment of Histology and Embryology Recep Tayyip Erdo?an UniversityFaculty of Medicine Department of Pharmacology Canakkale Onsekiz Mart University

daidzein, a plant extract, has antioxidant activity. It is hypothesized, in this study, that daidzein exhibits neuroprotective effects on cerebral ischemia. Rat models of middle cerebral artery oc- clusion were intraperitoneally administered daidzein. Biochemical and immunohistochemical tests showed that superoxide dismutase and nuclear respiratory factor 1 expression levels in the brain tissue decreased after ischemia and they increased obviously after daidzein administra- tion; malondialdehyde level and apoptosis-related cysteine peptidase caspase-3 and caspase-9 immunoreactivity in the brain tissue increased after ischemia and they decreased obviously after daidzein administration. Hematoxylin-eosin staining and luxol fast blue staining results showed that intraperitoneal administration of daidzein markedly alleviated neuronal damage in the ischemic brain tissue. These findings suggest that daidzein exhibits neuroprotective effects on ischemic brain tissue by decreasing oxygen free radical production, which validates the afore- mentioned hypothesis.

关键词： nerve regeneration brain ischemia stroke daidzein oxidative stress apoptosis superoxidedismutase malondialdehyde nuclear respiratory factor 1 neural regeneration

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Supplementing daidzein in diets improves the reproductive performance, endocrine hormones and antioxidant capacity of multiparous sows

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Animal Nutrition 2021年第4期7卷 1052-1060页

作者： Yan Li Guoru He Daiwen Chen Bing Yu Jie Yu Ping Zheng Zhiqing Huang Yuheng Luo Junqiu Luo Xiangbing Mao Hui Yan Jun He Institute of Animal Nutrition Sichuan Agricultural UniversityKey Laboratory of Animal Disease-Resistance NutritionMinistry of EducationChengdu 611130China New Hope Liuhe Co.Ltd. China

Certain hormones play important roles in modulating mammalian reproductive behaviour.daidzein is a well-known isoflavonic phytoestrogen that possesses oestrogenic activity.This study was conducted to probe the effects of daidzein supplementation in gestation diets on the reproductive performance in sows.A total of 120 multiparous sows(Landrace x Yorkshire)were randomly assigned to 2 groups(n=60)and fed either a base diet(control)or one containing 200 mg/kg daidzein during gestation.We discovered that daidzein supplementation significantly increased the total number of piglets born per litter and number of piglets born alive per litter(Pdaidzein-treated group than in the control group at35 d of gestation(Pdaidzein increased the serum SOD activity and total anti-oxidative capacity(T-AOC)at 85 d of gestation(Pdaidzein elevated the expression levels of the sodium-coupled neutral amino acid transporter 1(SLC38 A1)and insulin-like growth factor 1(IGF-1)genes in the placenta(Pdaidzein ingestion could improve sow reproductive performance by changing serum hormones,elevating anti-oxidative capacity and upregulating critical functional genes in the placenta.

关键词： daidzein Immunity Anti-oxidative Reproductive performance Sow

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Synthesis of Novel Phosphorylated daidzein Derivatives and ESI Investigation on Their Non-Covalent Complexes with Lysozyme

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Chinese Journal of Chemistry 2007年第7期25卷 1008-1013页

作者：陈晓岚石晓娜屈凌波袁金伟卢建莎赵玉芬 Key Laboratory of Chemical Biology and Organic Chemistry of Henan Province Department of Chemistry Zhengzhou University Zhengzhou Henan 450052 China Anyang Normal University Anyang Henan 455002 China The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education Department of Chemistry School of Life Sciences and Engineering Tsinghua University Beijing 100084 China

daidzein （7,4＇-dihydroxyisoflavone） was phosphorylated by a modified Atherton-Todd reaction. The structures of the five target product, were determined by X-ray, IR, NMR and ESI-MS. Electrospray ionization results show that in the gas phase all the phosphorylated daidzein derivatives could form non-covalent complexes with the protein lysozyme, while non-covalent complexes were not detected in the mixed solution of daidzein with lysozyme. Relative affinity of every non-covalent complex was obtained according to its different decomposition orifice voltage.

关键词： daidzein phosphorylation ESI-MS non-covalent complex

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Influence of dosage forms on pharmacokinetics of daidzein and its main metabolite daidzein-7-O-glucuronide in rats

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Acta Pharmacologica Sinica 2005年第9期26卷 1145-1152页

作者： Feng QIU~2 Xiao-yan CHEN~2 Bo SONG~2 Da-fang ZHONG~3 Chang-xiao LIU~4 2 Laboratory of Drug Metabolism and Pharmacokinetics,Shenyang Pharmaceutical University,Shenyang 110016,China Laboratory of Drug Metabolism and Pharmacokinetics Shenyang Pharmaceutical University Shenyang China Tianjin Key Laboratory of Drug Metabolism and Pharmacokinetics Tianjin Institute of Pharmaceutical Research Tianjin China

Aim:To investigate the influence of dosage forms on the pharmacokinetics ofdaidzein and its main metabolite daidzein-7-O-glucuronide in Wistar rats.Methods:After administration of two typical dosage forms(daidzein solution andsuspension),the concentrations of daidzein and daidzein-7-O-glucuronide weredetermined by an LC-MS-MS method.The pharmacokinetic parameters were cal-culated and analyzed statistically using the Student’s t-test.Results:Absorp-tion of daidzein after administration of daidzein solution(tmax=0.46 h)was morerapid than that of the suspension(tmax=5.00 h).The peak plasma concentrationsof daidzein after administration of daidzein solution and suspension were 601.1 μg/Land 127.3 μg/L,respectively,and those of daidzein-7-O-glucuronide were 3000 μg/Land 192.6 μg/L,respectively.The absolute bioavailabilities of free daidzein in ratsafter administration of daidzein solution and suspension were 12.8% and6.1%,respectively,which were calculated to be 47.0% and 12.2%,respectively,inthe form of total daidzein(free plus conjugated daidzein).Conclusion:Absorp-tion of daidzein solution was better than absorption of suspension(P<0.05).

关键词： daidzein daidzein-7-O-glucuronide influence dosage forms pharmacokinetics rats

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Dietary daidzein Enhances Antiapoptotic Effect of 17β-Estradiol (E_2) on Breast Cancer MCF-7 Cells

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Chinese Journal of Cancer Research 2010年第1期22卷 10-16页

作者： Xiao-fei Wu Yi-jing Wang Guo-liang Xia Mei-jia Zhang State Key Laboratory for Agrobiotechnology College of Biological Sciences China Agricultural University Beijing 100193 China

Objective： To investigate whether dietary daidzein interact with endogenous 17β-Estradiol （E2） to give rise to additive or inhibitory effects on proliferation and apoptosis in breast cancer cells. Methods： Cell cycle distribution and apoptosis induction were analyzed by using flow cytometry when breast cancer cell lines MCF-7 were cotreated with daidzein （1, 5 μmol/L） and E2 （0.1-10 nmol/L） for 5 days. Whether daidzein could alter E2-modulated mRNA expression of estrogen receptor alpha （ERα）, estrogen receptor beta （ERI3） and ERβ-estrogen response element （ERE） dependent transcription was investigated by RT-PCR and luciferase induction assays. The effects of daidzein on E2-modulated expression of proapoptotic p53, bax and antiapoptotic bcl-2 at both mRNA and protein levels were also investigated by RT-PCR and Western blot. Results： daidzein enhanced the antiapoptotic effect in an Ea dose-dependent manner, but had no effect on E2-induced proliferation. daidzein antagonized E2-induced ERβ mRNA expression and ERβ-ERE dependent transcription. In addition, daidzein only antagonized E2-upregulated expression of p53 and bax, but had no effect on E2-upregulated expression of bcl-2. Conclusion： daidzein enhances the antiapoptotic effect of E2 on breast cancer cells by inhibiting E2-mediated p53-bax proapoptotic pathway. These results suggest that dietary daidzein may enhance deleterious effect of endogenous E2 in hormone-dependent breast cancer.

关键词： daidzein E2 Breast cancer MCF-7 cells Antiapoptotic effect Estrogen receptor （ER）

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Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

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Progress in Natural Science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, SUI Senfang and YANG Mengsu (State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China) State Key Laboratory of Biomembrane Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Department of Chemistry College of Chemistry and Environmental Science Hebei University Baoding 071002 China Department of Biology and Chemistry City University of Hong Kong Hong Kong Hong Kong

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

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Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii

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Journal of Pharmaceutical Analysis 2011年第1期1卷 13-19页

作者： Ying-Kun Liu E Yan Han-Ying Zhan Zhi-Qi Zhang Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaan.ri Normal University) Ministry of Education Xi'an 710062 China Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang Forestry UniversityLin'an 311300China Department of Chemistry QinghaiNormal UniversityXining 810008China

Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials.

关键词： microwave-assisted extraction（MAE） response surface methodology（RSM） puerarin daidzein HPLC-fluorescence detection Radix Puerariae thomsonii

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Neuroprotective effect and mechanism of daidzein in oxygen-glucose deprivation/reperfusion injury based on experimental approaches and network pharmacology

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Traditional Medicine Research 2021年第5期6卷 10-19页

作者： Ming-Hua Xian Si-Kai Zhan Ke-Ning Zheng Qu-liu Ke-Ning Li Jia-Yin Liang Shu-Mei Wang Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangzhou 510006China Engineering&Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province Guangzhou 510006China School of Traditional Chinese Pharmacy Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Pharmacy Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Traditional Chinese Pharmacy China Pharmaceutical UniversityNanjing 211198China

Background:daidzein,phytoestrogens derived from the Pueraria lobata(Willd.)Ohwi root used in traditional Chinese medicine,has a wide range of biological activities,including antioxidant,anti-inflammatory,and neuroprotection.However,the neuroprotective role of daidzein in oxygen-glucose deprivation/reperfusion injury and its underlying mechanism are still unknown.Methods:In this study,we used pheochromocytoma cells induced by oxygen-glucose deprivation and reperfusion to study the potential effect in the protection of the nerve cells.Then,we used molecular docking simulation and network pharmacology to predict the possible targets and pharmacological pathways of daidzein.Western blot was used to verify the expression of target proteins with or without adding the inhibitors.Results:After daidzein treatment,cell vitality had an upward trend(Pdaidzein.Gene Ontology enrichment analysis indicated the pathways mainly contained molecule metabolic process,while Kyoto Encyclopedia of Genes and Genomes enrichment analysis focus on pathways in terms of inflammation such as tumor necrosis factor signal pathway.Then,Western blot results showed that daidzein had a significant increase on the expression of protein catalase(Pdaidzein reversed catalase level alterations after oxygen-glucose deprivation reperfusion injury in a dose-dependent manner which was consistent with the catalase antagonists-based experiments.Conclusion:Th

关键词： daidzein neuroprotection catalase oxygen-glucose deprivation and reperfusion network pharmacology

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Optimization of supercritical fluid extraction of daidzein from soybean after extrusion

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International Journal of Agricultural and Biological Engineering 2009年第2期2卷 57-64页

作者： Zhou Quancheng Li Lei Shen Dechao School of Light Industry and Agricultural Engineering Shandong University of TechnologyZibo 255091China

A set of optimal conditions for supercritical fluid extraction(SFE)of daidzein in soybean previously subjected to extrusion at the rotate speed of screw 4.5×g and the temperature of 80℃was developed.The quadratic orthogonal rotation design with five factors(extracting temperature,extracting time,ethanlol content,cosolvent quantity and flow rate)was used and a mathematical model was developed based the experimental results.Optimal conditions were verified and compared with control experiments conducted in parallel.The results from SFE showed that all five experiment factors had significant effects on the yield of daidzein.The mathematical model agreed well with the experimental results.It was found that there were 27 optimal extract conditions under which daidzein yield was higher than 0.092%.The optimal extracting conditions were stable and reproducible.Results from the control experiments showed that extrusion could significantly enhance the extraction of daidzein.The extraction of daidzein from extruded soybean and its products with supercritical CO_(2) is a potential technique for practical applications in the future.

关键词： extraction daidzein supercritical extrusion cosolvent mathematical model

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