Expressions of NG2 oligodendrocyte precursor cells in brain of rats during different postnatal developmental stages
会议名称：《中国神经科学学会第四次会员代表大会暨第七届全国学术会议》
会议日期：2007年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
基 金：supported by Nature Science Foundation of China No.30600678 and No.30670686
关 键 词：NG2 oligodendrocyte precursor cells(OPCs) immunohistochemisty development
摘 要：正Classic studies recognize two functionally segregated macroglial cell types in the central nervous system（CNS）, namely astrocytes and oligodendrocytes.A third macroglial cell type including a unique population of glial cells has now been identified by its specific expression of the NG2 chondroitin sulphate proteoglycan（NG2 glia） and described as oligodendrocyte precursor cells（OPCs）.This study was undertaken to determine when most of NG2 cells exhibit adult characteristics in the rat cerebral cortex and hippocampus and whether these populations are present from birth or appear later ***（PO）,postnatal day 7（P7）,P14,P21,P35,adult and old（more than 16 months） Sprague-Dawley rats were used in this *** each result at least 5 animals and 20 slices were used in five independent *** temporal distribution of NG2 cells in the rat brain during postnatal development was studied by immunohistochemistry as described ***2 cells in 20μm cryosection from cerebral cortex and hippocampus of rats in seven different developmental stages were counted by Image Pro Plus *** birth,NG2 cells are present throughout the cerebrum,hippocampus and dentate gyrus with the exception of the pyramidal cell layer in hippocampus and granule cell layer in dentate gyrus,in which rare NG2 cells intermingle with other cells in its deepest *** this stage,the majority of hippocampal and cerebral NG2 cells exhibit a stellate morphology,a small cell body with a little *** P7,the number of NG2 expressing cells increases remarkably with significant differences compared with *** P14 to P35,the distribution of NG2 cells is similar to that observed in *** populations of the NG2 immunostaining cells at P14,P21,P35,and adult rats decreases drastically compared with *** numerical cell density was counted,no significant differences were found each other. However,the morphology of NG2 cells is not the same in the white matter and in the grey *** th