Behavior and Functional Study of Myeloid Leukocytes in the Epimorphic Regeneration of Larval Tail Fin in zebrafish
会议名称：《2012全国发育生物学大会》
会议日期：2012年
学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖]
摘 要：Macrophages and neutrophils are the pivotal immune phagocytes that enter the wound after tissue injury to remove the cell debris and invaded microorganisms,and facilitate the regrowth of injured *** advantage of the regeneration abilities of zebrafish and the newly generated Tg(corola:eGFP/lyz:Dsred) zebrafish line with labeling of both myeloid lineages,we assessed the behaviors and functions of neutrophils and macrophages during tail fin *** imaging showed that within 6 hours post amputation(hpa)-the inflammatory stage,neutrophils with high mobility were the primary cells scavenging apoptotic bodies and small cell debris,although they had limited phagocytic capacity and quickly underwent *** of interleukin factor could inhibit the directional migration of neutrophils but not macrophages to the wound region,suggesting different molecules responded to the distinct behaviors of both cell types during this *** 6 hpa onwards-the resolution and regeneration stage,macrophages became the dominant scavengers, efficiently resolving inflammation and facilitating tissue remodeling and *** of macrophages but not neutrophils impaired the regeneration,resulting in the formation of large vacuoles in the regenerated fins. Conclusions:Our study documents the differing behaviors and functions of macrophages and neutrophils during tissue *** the future,we will explore the detailed mechanisms used by both cell types in the whole process