Cubic Lipid Phase Crystallization of Membrane Proteins
作者单位：University of CaliforniaIrvineCA92697-3900USA University of CaliforniaIrvineCA92697-3900USA University of CaliforniaIrvineCA92697-3900USA
会议名称：《10～(th) International Conference on the Crystallization of Biological Macromlecules》
会议日期：2004年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
摘 要：正 Cubic Lipid Phase （CLP）: The CLP method for membrane protein crystallization has been refined to allow large-scale screening of various membrane proteins. The various parameters （CLP lipid, water content, bilayer lipid additive, pH, ionic strength, precipitating agent etc.） can be varied. Three distinct seven-transmembrane proteins where crystallized and their structures *** （BR）: High-resolution maps from X-ray diffraction of bacteriorhodopsin crystal obtained in CLP and some of its photointermediates have yielded insights to how the isomerization of the bound retinal drives ion transport.