DC-UbP/UBTD2,a scaffold protein involved in orchestrating ubiquitination pathway
作者单位：State Key Laboratory of Molecular BiologyInstitute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences
会议名称：《中国生物化学与分子生物学会第十届会员代表大会暨全国学术...》
会议日期：2010年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学]
摘 要：DC-UbP,also known as UBTD2,was first identified from dendritic cells.It is a ubiquitinlike(UbL) protein containing a UbL domain in the C-terminus.We have determined the solution NMR structures of the N-terminal ubiquitinbinding domain(UBD) and the Cterminal UbL domain of DC-UbP.By pull-down and mass spectrometric analysis, we identified that DC-UbP can interact with both ubiquitin activating enzyme(UbEI) and a ubiquitin specific protease (USP5).UbEI plays an important role in the first step of protein ubiquitination,whereas USP5(also named IsoT) is