Dicerl and miR-9 are required for proper Notchl signaling and the Bergmann glial phenotype in the developing mouse cerebellum
作者单位：West China Developmental c& Stem Cell Institute Sichuan University School of Life Science Sichuan University Department of Obstetric & Gynecologic and Pediatric Sichuan University Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects Ministry of Education West China Second University Hospital Sichuan University
会议名称：《中国神经科学学会第十届全国学术会议》
会议日期：2013年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：hGFAP Dicer Bergmann glia Notchl miR-9
摘 要：MicroRNAs（miRNAs） have important roles in the development of the central nervous system（CNS）.Several reports indicate that tissue development and cellular differentiation in the developing forebrain are disrupted in the absence of ***,the functions of miRNAs during cerebellar development have not been systematically ***,we conditionally knocked out the Dicerl gene under the control of the human glial fibrillary acidic protein（hGFAP） promoter to examine the effect of miRNAs in the developing *** particularly focused on the phenotype of Bergmann glia（BG）.The hGFAP-Cre activity was detected as early as embryomc day 13.5（E13.5） at the rhombic lip（RL） in the cerebellar plate,and later in several postnatal cerebellar cell types,including *** ablation induces a smaller and less developed cerebellum,accompanied by aberrant BG *** signaling appears to be blocked in Dicerl-ablated BG,with reduced expression of the Notchl target gene,brain lipid binding protein（BLBP）.Using neuronal co-culture assays,we showed an intrinsic effect of Dicerl on BG morphology and Notchl target gene *** further identified miR-9 as being differentially expressed in BG and showed that miR-9 is a critical,but not the only,miRNA component of the Notchl signaling pathway in cultured BG cells.