A Simple Method for Depletion High-abundant Proteins and Enriching Specific Proteins for Human Proteomic Analysis

作     者：Y.S. Ning, Y.Li, C.L.Luo, W.N.SITU, WB..Hao, N.Li, Y.D. Wang, X. L.Liu, L.H. Wang, Y Xue, F..C He, Q.H. Sun, M. Li (The First Military Medical University, Guangzhou 510515 Beijing Institute of Radiation Medicine, Beijing 100850 United Antibody Center. Shanghai 200092 , China) 

会议名称：《中国蛋白质组学首届学术大会》

会议日期：2003年

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

摘      要：Two-dimensional electrophoresis (2-DE) is a valuable tool for the separation and characterization of pro-teins from complex biological samples. One of the main limitations of 2-DE is its inability to visualize low-abundant proteins, particularly in the presence of highly abundant proteins. In plasma, a few high-abundantproteins, such as albumin, immunoglobulins, lipoproteins, fibrinogen, et al, accounts for approximately 90%of the total protein. As protein expression within plasma has such a large dynamic range, attempts to detect traceproteins by increasing the total amount of plasma loaded onto a 2-DE gel, means excessive amounts of predomi-