Transgene expression of enhanced green fluorescent protein in cloned rabbits generated from in vitro-transfected adult fibroblasts
会议名称：《第九届中国实验动物科学年会（2010新疆）》
会议日期：2010年
学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖]
摘 要：Objective Live rabbits have previously been generatedthrough nuclear transfer using adult cells as nuclear *** demonstrated in this study that transfected adult rabbit fibroblasts are also capableof supporting full-term *** fibroblasts were transfected with a pEGFP-C1 plasmid using Lipofectamine TM 2000,and the transgenic cells were derived from conditioned *** transgenic fibroblasts were cultured until confluent and then serum-starved prior to be used as nuclear *** nuclear transfer and activation,22%(12/55) of the transgenic cloned embryos developed to the blastocyst stage.A total of 114 embryos at the 4- to 8-cell stage were transferred to the oviducts of 8 pseudo-pregnant mothers;5 of these animals became pregnant,and 3 of the 5 mother rabbits carried the pregnancy to term. Caesarean section was performed on the 3 pregnant mothers,yielding 4 kits,one of which has survived for more than 9 *** fluorescence could be detected in the toenails of the living cloned rabbit and the offspring from the living cloned rabbit under ultraviolet *** analyses confirmed that all 4 cloned rabbits were genetically identical to the transgenic donor cells,and that they all carried the EGFP *** present study demonstrated that transgenic rabbits can be generated through nuclear *** results may facilitate future developments in the genetic engineering of rabbits.