The effect of osmotic pressure to aquaporin gene Glp-F expressed by ***

作     者：Changqing Li~(1,3),Zhibang Yang~(1,2),et al (1.Department of pathobiology and Immunology Experimental Teaching Center of Bacic Medicine,2.Institute of Neuroscience,Chongqing university of medical sciences,Chongqing 400016 3. Department of analysis Sixth People’s Hospital Of Chengdu,Chengdu,610051) 

会议名称：《重庆微生物学会第九届会员代表大会暨学术年会》

会议日期：2009年

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

关 键 词：degenerate PCR RT-PCR Glp-F aquaporin 

摘      要：Objective:*** growth in Nutrient Agar which have different osmotic conditions, determinate OD600 at 24,48,72,96 *** reverse transcription assay was used to quantify the levels of Glp-F mRNA in different osmolality ***:Basic media made as Nutrient Agar. Media-N were made hypertonic by adding NaCl to increase the concentrations up to 125,250,500 or 750 ***-N were made hypotonic by diluting them with water(50%and 33%).Media-H were made hypertonic by adding NaCl and HgCl