houStudies on the effect ofalginate-chitosansustained releasemicrocapsules fortranshepatic arterial embolization in VX2 rabbit liver cancer model
会议名称：《加快转变医药发展方式，占领科学技术制高点——2011年中国药学大会暨第11届中国药师周》
会议日期：2011年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：embolization VX2 necrosis microsphere NCTD alginate chitosan
摘 要：Purpose:To study the preparation technique of a sustained-released microsphere (LSD/NTCD-ACM) and evaluate its characteristic and effects for transarterial hepatic chemoembolization (TACE) in an animal model of liver cancer (VX2).Mmethods:We prepared LSD/NTCD-ACM through the emulsification-gelation method with liquid paraffin as oil phase,the hybrid of chitosan and sodium alginate as water phase,Span-80 as emulsifier,and glutaraldehyde as cross linking *** its in vitro release in pH 7.4 PBS by dynamic dialysis *** 24 male New Zealand white rabbits were randomly divided into four groups with 6 rabbits assigned to each ***2 carcinoma tissues were implanted into the left hepatic lobe using a 16g biopsy *** tumors were allowed to grow for weeks,and studies were performed until the volume of the tumors detected by ultrasonograph reached 2-3 cm *** anesthesia,transcatheter hepatic arterial embolization was performed and LSD/NTCD-ACM(60-120μm),LSD/NTCD-ACM(120-200μm) or NCTD solution was injected into the hepatic arteries of animals in the three treatment groups and the left one is control group(with no treatment).Before and at 3,7,14 days after injection,the volume of the tumor was measured by ultrasonograph,the serum of all rabbits was collected,and the level of aspartate aminotransferase (AST) was *** the last course of their disease,the rabbits were given analgesics to relieve *** survival period of the three groups of rabbits after treatment was recorded,and degree of liver cell necrosis was also assessed on the histopathological ***:The drug entrapment efficiency (EE%) of LSD/NTCD-ACM(60-120μm) was 74.62% and drug-loading (DL%) amount was 7.46% while 86.93% for EE and 9.23%for DL of LSD/NTCD-ACM (120-200μm).Both the two particles could release as long as 20 days in pH 7.4 *** tumor growth rate in the LSD/NCTD-ACM (60-120/μm) group (7.76±0.41,tumor volume from2.48±0.25 to19.24±1.33 cm 3) and in the