Acetylation of Osx enhances its protein stability,DNA binding ability and transcriptional activity and is required for osteoblast differentiation
作者单位：Department of Developmental GeneticsNanjing Medical University Department of Laboratory Medicinethe First Affiliated Hospital of Nanjing Medical University Molecular Endocrinology LaboratoryDepartment of EndocrinologyOdense University Hospital
会议名称：《第十四次全国医学遗传学学术会议》
会议日期：2015年
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
关 键 词：Osterix acetylation CBP osteoblast differentiation
摘 要：Osterix(Osx),a C2H2-type zinc-finger containing transcription factor,plays an essential role in controlling osteoblast differentiation and bone *** precise molecular regulation of Osx expression is still not fully ***,we studied the post-translational regulation of Osx by *** found that both endogenous and exogenous Osx protein increased after treatment with histone deacetylase inhibitors Trichostatin A(TSA)and hydroxamic acid(SAHA)in *** the results of Immunoprecipitation(IP)indicated that Osx was an acetylated protein and CREB binding protein(CBP),and less efficiently p300,acetylated *** interaction and colocalization of CBP and Osx were demonstrated by Co-IP and immunofluorescence(IF),*** addition,K307 and K312 were identified as the acetylated sites of *** the other hand,HDAC4,a histone deacetylase(HDAC),was found to interactand be co-localized with ***4 was demonstrated to mediate the deacetylation of ***,we found that acetylation of Osx enhanced its protein stability,DNA binding ability and transcriptional ***,we demonstrated that acetylation of Osx was required for the osteogenic differentiation of C2C12 *** together,our results provide evidence for the first time that CBP-mediated acetylation and HDAC4-mediated deacetylation take a critical role in the modification of Osx,thus play an important role in osteoblast differentiation.