The R5Y5 sequence's affinity to histones in vitro
作者单位：School of MathematicsPhysics and Biological EngineeringInner Mongolia University of Science and Technology
会议名称：《第五届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会高通量时代的生物信息学与系统生物学》
会议日期：2012年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
关 键 词：nucleosome positioning R5Y5 DNA sequence epigenetic
摘 要：Background:Nucleosome positioning plays an important role in regulation of the gene activity in eukaryotic *** sequence is believed to be one of the major factors affecting the nucleosome positioning and sequence-dependent nucleosome positioning is largely dependent on DNA deformability *** of basic binary periodical patterns was well established:in purine/pyrimidine alphabet YRRRRRYYYYYR by theoretical calculation model,but its validity need to be further verified. Methods:One of chemical synthetic R5Y5 sequences version was cloned into multiple cloning sites of *** then the sequence was labeled by biotin and purified after PCR. A salt dialysis method in vitro was employed to assembly nucleosome and in which 601 sequences was used as control. Results:The sequence has strong nucleosome formation ability in the 1.2 ratio of octamer to *** in the 0.8 ratio it has low affinity to ***,its stability to assembly nucleosome is not as high as 601. Conclusions:The R5Y5 sequence’s ability to assembly nucleosome was perhaps strong in vitro and the result was the basic to further investigate the sequence-dependent of nucleosome positioning.