Dexmedetomidine reverses peripheral inflammation-induced acitivation of astrocytes in the spinal cord
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：dexmedetomidine astrocyte microglia monoarthritis thermal hyperalgesia rat
摘 要：正Objective Spinal glia is of the utmost importance in developing and maintaining allodynia and hyperalgesia following tissues inflammation or *** systemically and intrathecally administered dexmedetomidine exhibited significant analgesic effect in human with inflammatory disease and animals with ***,whether the spinal glial functional states are involved in the analgesic effect of dexmedetomidine is still *** present study investigated the effects of systemic repeated administration of dexmedetomidine on thermal hyperalgesia and spinal glial activation evoked by monoarthritis（MA）.Methods Monoarthritis was induced by an intra-articular injection of Complete Freund’s Adjuvant（CFA）.Paw withdrawal latency（PWL） by radiant heat was *** analyzed cell markers GFAP for astrocytes and Iba-1 for microglia in the spinal ***（1） Extensive activation of astrocytes and microglia were found in the spinal dorsal horn of MA rats.（2） Repeated intraperitoneal（i.p.） administration of dexmedetomidine（10μg/kg） significantly attenuated MA-induced thermal hyperalgesia and suppressed spinal astrocytic but not microglial activation.（3） Double immunofluorescence showed that a2ARs immunoreactivities were widely colocalized with GFAP,an astrocytic marker,but not with Iba-1,a microglial marker,in the spinal *** Blockade of spinal astrocytic activation was involved in anti-nociceptive mechanisms of dexmedetomidine.