Phytochemical isolation, antimicrobial activity and pharmacological studies of Clausena dentata (willd.) roem.
作者单位：Centre for Advanced Studies in Botany University of Madras Guindy campus Chennai -600 025 India. College of Pharmacy Sri Ramachandra Medical College and Research Institute(Deemed University) Porur Chennai -600 116 India Bio-organic Division Baba Atomic Research Centre Trombay Mumbai -400 085 India. Bio-organic Division Baba Atomic Research Centre Trombay Mumbai -400 085 India.
会议名称：《The 3～(rd) International Conference on Natural Products :Natural Products -a Must for Human Survival ABSTRACTS》
会议日期：2004年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
摘 要：The major advantage of natural products for random screening is the structural diversity provided by most available combinatorial approaches based on heterocyclic compounds. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs, mainly growing Indo-Malayan with a few in China, Africa and Australia. Ten species are known to grow in India, of which 5 are of economically importance. C. dentata, a plant of immense medicinal value was taken for detailed study for phytochemical, antibacterial and pharmacological investigations. The dry powder of the stem bark was extracted successively with 3 solvents viz., hexane, chloroform and methanol. The extracts were subjected to column chromatography. Four compounds were isolated. Two coumarins, 3-(1,1-dimethylallyl) xanthyletin and dentatin were isolated from hexane extract. The chloroform extract yielded another coumarin, nordentatin. An alkaloid, carbazole was isolated from methanol extract. All the compounds were identified based on spectral studies such as GC, UV, IR, NMR and MS. This investigation is the first report of the dentatin and nordentatin stem bark of C. dentata. The 13C-NMR studies of dentatin and nordentatin have been carried out for the first time. The existence of carbazole alkaloid is reported for the first time in C. dentata. The compounds showed significant antibacterial activity against E. coli, Salmonella typhi, Klebsiella pneumoniae, Vibrio cholerae and Staphylococcus aureus. The activity by dentatin and nordentatin against Vibrio cholerae was comparable with standard antibiotic ciprofloxacin. All the 3 extracts and the 4 isolated compounds were tested for various pharmacological activities such as anti-inflammatory, analgesic and hepatoprotective effect for extracts, anticoagulant, antiplatelet aggregation, prothrombin time and effect on perfused frog heart for isolated compounds. The present study is the first report for pharmacological activities of C. dentata.