The study of anti-LPS material basis and biological activity within Allium Sativum L.
会议名称：《第十五届国际药理学大会》
会议日期：2006年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Allium sativum L. biosensor Lipopolysaccharide Lipid A sepsis
摘 要：正 Objective: We screened the active components extreated from Allium Sativum L., and studied their biological activities against ***:（1） The active fractions were isolated by biosensor technique. （2） Observing the inhibition of TNF -α and IL - 6 release in RAW264.7 cells induced by LPS.（3） Observing the protection of the fraction for mice from lethal challenge of LPS.（4） Isolating the active monomes from ASLA. studing their LPS - neutralizing effect. Results : （1） The active fraction with the best affinity was separated and benamed of AlliumSativum L. fraction A （ASLA）.（2） ASLA could markedly inhibite TNF-αand IL-6 release in RAW264.7 cells induced by LPS;It also protected mice from lethal challenge of LPS. （3） There were two main components in ASLA. Both of them had significant biological activities against sepsis. Conclusions: （1） The ASLA had significant activity aganst sepsis.（2） The main components of ASLA were two monomers. And theyhad significant activities against sepsis.