Spectroscopic and electrochemical studies of interaction between Azorubine and CT-DNA
作者单位：Department of Plant BiologyFaculty of natural ScieneceUniversity of Tabriz Department of Animal BiologyFaculty of natural ScieneceUniversity of Tabriz
会议名称：《International Conference on Computer Science and Information Technology(ICCSIT’2011);International Conference on Electrical,Electronics and Civil Engineering(ICEECE’2011);International Conference on Mechanical,Production and Automobile Engineering(ICMPAE’2011);International Conference on Humanities,Geography and Economics(ICHGE’2011);International Conference on Chemical,Ecology and Environmental Sciences(ICCEES’2011);International Conference on Medical,Biological and Pharmaceutical Sciences (ICMBPS’2011)》
会议日期：2012年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
关 键 词：Azorubine DNA interaction Food additive Outside binding.
摘 要：The interaction of native calf thymus DNA （CT-DNA） with azorubine in 10 mM Tris-HCl aqueous solution at neutral pH 7.4 was monitored by UV-vis absorption and voltammetric techniques. Upon addition of the azorubine, UV absorption band of DNA decreased. After addition different concentrations of CT-DNA, UV absorption band of azorubine showed hyperchromisity. The binding constants （K b ） for the azorubine with DNA was estimated to be 6.2×104M-1 . Electrochemical interaction of azorubine with ctDNA showed decrease in both peak current and no detectable potential shift was observed. The experimental results suggest that azorubine binds to DNA via an outside binding mode.