C-terminal domain of ICA69 interacts with PICKl and acts on trafficking of PICK1-PKCα complex and cerebellar plasticity
作者单位：Department of NeurobiologyKey Laboratory of Medical Neurobiology of the Ministry of HealthZhejiang Province Key Laboratory of NeurobiologyZhejiang University School of Medicine Department of NeurobiologyCenter of Scientific TechnologyCranial Cerebral Disease LabNingxia Medical University
会议名称：《中国神经科学学会第十届全国学术会议》
会议日期：2013年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：ICA69 PICK1 PKCa plasticity traffickin
摘 要：Objective The role of C-terminal Domain of ICA69（ICAC） in regulating trafficking of of PICK1-PKCa Complex and Cerebellar Plasticity remains *** experiments try to identify how the ICAC of ICA69 affect PICK1 trafficking and induction of long-term *** The interaction between ICA69 or ICAC and PICK1 was identified by co-immunoprecipition,immunostaining and FRET *** ICAC domain of ICA69 affects binding of PICK1-PKCa was measured by Time-lapse imaging *** proteins affects induction of long-term depression of Purkinje cells by electrophysiology *** ICAC inhibited the trafficking of PICK1 to the plasma membrane mediated by activated PKCa and the induction of long-term depression in cerebellar Purkinje *** ICAC is an important domain for ICA69-PICK1 interaction and plays an important role in PICK1-mediated neuronal plasticity.