Resveratrol inhibits β-amyloid oligomeric cytotoxicity but does not prevent oligomer formation
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Alzheimer’s disease β-amyloid resveratrol Thioflavin T MTT aggregation destabilization oligomer
摘 要：正β-Amyloid（Aβ） aggregation has been strongly associated with the neurodegenerative pathology and a cascade of harmful events related to Alzheimer’s disease（AD）.Inhibition of Aβassembly,destabilization of preformed Aβaggregates and attenuation of the cytotoxicity of Aβoligomers and fibrils could be valuable therapeutics of patients with *** studies suggested that moderate consumption of red wine and intake of dietary polyphenols,such as resveratrol,may benefit AD phenotypes in animal models and reduce the relative risk for AD clinical *** understand the mechanism of this neuroprotection,we studied the effects of resveratrol,an active ingredient of polyphenols in wine and many plants,on the polymerization of Aβ42 monomer,the destabilization of Aβ42 fibril and the cell toxicity of Aβ42 in vitro using fluorescence spectroscopic analysis with thioflavin T（ThT）,transmission electron microscope （TEM）,Circular Dichroism（CD） and MTT *** results showed that resveratrol could dose-dependently inhibit Aβ42 fibril formation and cytotoxicity but could not prevent Aβ42 *** studies by Western-blot,dot-blot and ELISA confirmed that the addition of resveratrol resulted in numerous Aβ42 oligomer *** conjunction with the concept that Aβoligomers are linked to Aβtoxicity,we speculate that aside from potential antioxidant activities, resveratrol may directly bind to Aβ42,interfere in Aβ42 aggregation,change the Aβ42 oligomer conformation and attenuate Aβ42 oligomeric cytotoxicity.