Deciphering the mechanisms of phosphoryl transfer enzymes
作者单位：Krebs InstituteThe University of SheffieldSheffield S10 2TN Manchester Interdisciplinary BiocentreThe University of ManchesterManchester M1 7DNUK
会议名称：《第六届全国化学生物学学术会议》
会议日期：2009年
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
摘 要：正Using a combination of NMR spectroscopy,high resolution X-ray crystallography and computational chemistry we are able to examine the conformational behaviour of proteins under a very wide range of conditions.I will focus on the example of phosphoglycerate kinase（PGK） to illustrate what happens during domain folding,during assembly of the native enzyme,during substrate binding,and during transition state