Determination of Ergosterol in House Dust by HPLC
会议名称：《第四届全国环境与职业医学研究生学术研讨会》
会议日期：2005年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100402[医学-劳动卫生与环境卫生学] 10[医学]
基 金：National Science Fund(No.20277007)
关 键 词：house dust ergosterol HPLC GC-MS
摘 要：[Objective] A high performance liquid chromatographic (HPLC) method was developed for the determination of ergosterol in house dust. [Methods] The house dust was filtrated with a 200-mesh-filter. Samples were extracted by ultrasonic blending, filtration, refluxing, saponification and evaporation. Chromatography was carried out on Inertsil ODS column with acetonitrile as mobile phase pumped at flow rate of 1.0 mL/min. [Results] The limit of detection was 1.26 ×10-6mol/L. A linearity was observed in the range of 5.04 ×10-5 to 2.02 ×10-3 mol/L, with correlation coefficient as 0.9992. The intra- and inter-day relative standard deviation (RSD) were in the range of 10.2-11.7% and 4.54-7.34%, respectively. The absolute recoveries ranged between 77.1 and 80.1%. [Conclusion] The method was simple, sensitive, accurate and feasible. It can be used to quantify environmental ergosterol. Followed by gas chromatography (GC)-mass spectrometry (MS), the results were double- checked.