中药海马的鉴定及抗骨质疏松活性研究

作     者：王晓钰 

作者单位：福建中医药大学 

学位级别：硕士

导师姓名：黄宝康

授予年度：2015年

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主      题：中药海马 分子鉴定 抗骨质疏松活性 质量评价 高效薄层色谱 动物模型 

摘      要：Hippocampus, belonging to Syngnathidae family,which distributed widely in the shallow waters of tropical or subtropical, is an important small marine bony fish. Modern medical research have proved that hippocampus has anti-tumor, analgesic, anti-aging, anti-fatigue, anti-thrombotic and sex hormone-like effects and so *** is found by our survey that the market circulation of hippocampal medicinal market is relatively chaos and their quality is *** phenomena of shoddy and varieties ignorance are existd. Hippocampus histris and hippocampus spines is a confusing situation, most of the literature hardly make a clear distinction between them. Based on this, it is necessary to establish a scientific identification method of traditional Chinese medicine （TCM） hippocampus by their chemical or molecular biological information. Accurate identification, quality evaluation and control are of great *** is considered that hippocampus possess effects of warming impotence, scattered quarter swelling in Chinese medicine. Hippocampus folk usage was to treat injury or flutter Warming impotence,suggesting that the hippocampus may be related to *** previous experiments and literature confirms that, so we focused on HPTLC identification based on small molecule sterol compounds of traditional Chinese medicine （TCM） hippocampus and molecular identification research based on mitochondrial COI and Cytb gene, as well as activity of anti-osteoporosis study based on cytology in vitro experiment.1. Identification of the TCM Hippocampus by HPTLCWith 10 different regions of hippocampal medicinal materials as the research object, TLC identified by different extraction methods, extraction solvent, spotting amount, chromogenic agent, developing solvent, TLC plates and other conditions, we finally chose Cholest-4-en-3-one, cholesterol for reference, as wellas five hippocampus for reference drug, we chose HPTLC Silcon Gel 60 TLC plate, developing solvent system pet