EXPERIENCE ON HLA TYPING FOR ALLOGENEIC MARROW TRANSPLANTATION IN 217 MEMBERS OF 30 FAMILIES

作     者：王耀平 朱亚忠 钱龙华 应大明 

作者机构：Shanghai Institute for Pediatric ResearchSSMU 

出 版 物：《Medical Bulletin of Shanghai Jiaotong University》 (上海交通大学学报（医学英文版）)

年 卷 期：1993年第16卷第1期

页      面：56-62页

学科分类：10[医学] 

主　　题：typing of histocompatibility bone marrow transplantation mixed lymphocyte culture 

摘      要：From April 1978 to Oct 1990, 217 members of 30 families who requiredallogeneic marrow transplantation were examined for HLA typing, with HLA standardantiserum and mixed lymphocyte culture (MLC). The following conclusions could bedrawn: (1) when the HLA-A, B, C loci between donor and recipient were identical, theD locus also was generally identical too, but, in some rare cases the HLA-A, B, C wereidentical while the D locus remained different detected by MLC SI which wassignificantly increased; (2) in addition to twin, the sib were the main candidates of donorsin allogeneic marrow transplantation, however, if the phenotype of children was compati-ble to that of their parents with no significant stimulation in MLC, the parents could alsobe considered as a donor; (3) if the HLA-A, B, C typing was identical between fatherand motaher, the parents can also be considered as an eligible donor. Using HLA-A, B,C, DR and D loci identical sibs as donor, 9 of 11 cases (aplastic anemia 9 cases andANLL-M2 2 cases who received allogeneic marrow transplantation) had the evidence ofengraftment. Much attention should be paid to the difference of the minorhistocompatibility antigens, such as sex associated antigen between donor and recipient, be-cause even in 11 cases with HLA-A, B, C, DR and D loci identical sibs as donors formarrow transplantation, 5 cases still had the manifestation of GVHD in various degree,4/5 died of severe GVHD.