Bilayer nicorandil-loaded small-diameter vascular grafts improve endothelial cell function via PI3K/AKT/eNOS pathway

作     者：Zheng Xing Chen Zhao Chunchen Zhang Yubo Fan Haifeng Liu Zheng Xing;Chen Zhao;Chunchen Zhang;Yubo Fan;Haifeng Liu

作者机构：Key Laboratory for Biomechanics and Mechanobiology of Ministry of EducationSchool of Biological Science and Medical EngineeringBeijing Advanced Innovation Centre for Biomedical EngineeringBeihang UniversityXue Yuan Road No.37Haidian DistrictBeijing 100191China School of Pharmaceutical SciencesTsinghua UniversityBeijing 100084China Key Laboratory of Biomedical Engineering of Ministry of EducationZhejiang UniversityHangzhou 310027China Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness AppraisalZhejiang UniversityHangzhou 310027China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2021年第4卷第1期

页      面：72-86页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：This work was supported by the National Natural Science Foundation of China(31771058,32071359,11421202,61227902 and 11120101001) NationalKey Technology R&D Program(2016YFC1100704,2016YFC1101101) International Joint Research Center of Aerospace Biotechnology and Medical Engineering from Ministry of Science and Technology of China 111 Project(B13003) Research Fund for the Doctoral Program of Higher Education of China(20131102130004) Fundamental Research Funds for the Central Universities 

主　　题：Bilayer vascular grafts Nicorandil Sulfated silk fibroin Endothelial cell function PI3K/AKT/eNOS pathway 

摘      要：For the surgical treatment of cardiovascular disease(CVD),there is a clear and unmet need in developing small-diameter(diameter6 mm)vascular *** our previous work,sulfated silk fibroin(SF)was successfully fabricated as a potential candidate for preparing vascular grafts due to the great cytocompatibility and ***,vascular graft with single layer is difficult to adapt to the complex internal *** this work,polycaprolactone(PCL)and sulfated SF were used to fabricate bilayer vascular graft(BLVG)to mimic the structure of natural blood *** enhance the biological activity of BLVG,nicorandil(NIC),an FDA-approved drug with multi-bioactivity,was loaded in the BLVG to fabricate NIC-loaded *** morphology,chemical composition and mechanical properties of NIC-loaded BLVG were *** results showed that the bilayer structure of NIC-loaded BLVG endowed the graft with a biphasic drug release *** in vitro studies indicated that NIC-loaded BLVG could significantly increase the proliferation,migration and antioxidation capability of endothelial cells(ECs).Moreover,we found that the potential biological mechanism was the activation of PI3K/AKT/eNOS signaling ***,the results effectively demonstrated that NIC-loaded BLVG had a promising in vitro performance as a functional small-diameter vascular graft.